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VisualProtein//1 kit 500mL Solution X 2/BP01-1L

BlockPRO™BlockingBuffer

Ready-to-UseBlockingBufferforProteinDetection

BlockPRO™BlockingBufferisstitableforblockinginWesternblot,ELISA,immunohistochemistryandotherimmunochemicalapplication.Itcanblockexcessbindingsitebutnotcoveronthebindingproteinandthereforeincreasethesignalintensity.PresentbetterdataresultthanmilkandBSAblockingbuffer.

SpecialCharacteristic

  • Highefficiency:Increasesignalintensity

  • Highcompatibility:SuitablefordownstreamPBSTandTBSTwashprocedureandalsocompatIBLewitheveryimmunochemicalapplication

  • Simple:Safeandreadytouse

OrderInformation

Cat.No.ProductNameDescription

BP01-1L

BlockPRO™BlockingBuffer

1kit

500mLSolutionX2

ProductDetail

Figure1.SignalstrengthcomparisonofCasein,BSA,milk,andBlockPRO™BlockingBuffer.LoADIng30μgcelllysate(HepaG2)anddetectwithanti-AMPK(mouse,1:1,000).Secondaryantibody:anti-mouseIgG-HRP1:10,000.Membrane:Hyond™P.Detection:Hyperfilm™ECL.Allresultswereexposedfor30secondsandcapturebyX-rayfilm.

Figure2.BlockingwithskimmilkorBlockPRO™BlockingBufferanddetectbySEM.30μgcelllysate(HepaG2)separatedby12.5%SDS-PAGEandblockingbyskimmilkorBlockPRO™BlockingBuffer.MembranesdetectedbySEM.TheSEMresultshowedthatBlockPRO™BlockingBufferonlyblockingonexcessbindingsiteandrevealthepositionofantigenandthereforewillnotreducethesignalintensity.

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