BlockPRO™BlockingBufferisstitableforblockinginWesternblot,ELISA,immunohistochemistryandotherimmunochemicalapplication.Itcanblockexcessbindingsitebutnotcoveronthebindingproteinandthereforeincreasethesignalintensity.PresentbetterdataresultthanmilkandBSAblockingbuffer.
Highefficiency:Increasesignalintensity
Highcompatibility:SuitablefordownstreamPBSTandTBSTwashprocedureandalsocompatIBLewitheveryimmunochemicalapplication
Simple:Safeandreadytouse
Cat.No. | ProductName | Description |
BP01-1L | BlockPRO™BlockingBuffer | 1kit 500mLSolutionX2 |
Figure1.SignalstrengthcomparisonofCasein,BSA,milk,andBlockPRO™BlockingBuffer.LoADIng30μgcelllysate(HepaG2)anddetectwithanti-AMPK(mouse,1:1,000).Secondaryantibody:anti-mouseIgG-HRP1:10,000.Membrane:Hyond™P.Detection:Hyperfilm™ECL.Allresultswereexposedfor30secondsandcapturebyX-rayfilm.
Figure2.BlockingwithskimmilkorBlockPRO™BlockingBufferanddetectbySEM.30μgcelllysate(HepaG2)separatedby12.5%SDS-PAGEandblockingbyskimmilkorBlockPRO™BlockingBuffer.MembranesdetectedbySEM.TheSEMresultshowedthatBlockPRO™BlockingBufferonlyblockingonexcessbindingsiteandrevealthepositionofantigenandthereforewillnotreducethesignalintensity.
MSDS
Manual
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