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VisualProtein//About 100 membranes drawing/LH03-10

LuminolPen™,HRPSystem

Findyourdisappearmolecularweight

LuminolPen™,HRPSystemisaperfecttoolforchemiluminescentdetectioninWesternblot.Withsimplywritingonthepre-stainedMarker,thepositionofthemolecularweightcanbeshowedontheimage.ItcouldalsohelpyoutonotetheexperimentconditionorevaluatetheefficacyofECLsubstrate.

SpecialCharacteristic

  • Unique:theworld"sfirstluminescentcolordevelopmentmarkerpen
  • HelpyoutoevaluatetheefficacyofyourECLsubstrate
  • Convenient:abletomakenoteandshowontheimagewiththedataresult
  • CompatIBLe:applicablewithmostbrandsofECLsubstrate

OrderInformation

Cat.No.ProductNameDescription

LH03-50

LuminolPen™,HRPSystem

About1,000membranesdrawing

LH03-10

LuminolPen™,HRPSystem

About100membranesdrawing

ProductDetail

Figure1.LabelingmolecularweightandnoteonthemembranewithLuminolPen™.(A)and(C)aregeneralWesternblotchemiluminescencedetectionimage;(B)and(D)aretheimageusingLuminolPen™toindicatethepre-stainedmarker.

Figure2.Distinguishtargetsignalandnon-specificbindingsignalwithLuminolPen™.30μgcelllysateSP-1anddetectwithanti-ADNP(mouse,1:2,000).Secondaryantibody:anti-mouseIgG-HRP(1:15,000).Membrane:Hyond™P.Detection:Hyperfilm™ECL.Signalexposedfor30secondsanddetectedbyX-ray.ThemolecularweightnotebyLuminolPen™revealthecorrecttargetsignal(ADNP124KDa)onX-rayfilm.

Figure3.EvaluateECLsubstrateefficacybyLuminolPen™.30μgcelllysateHepaG2anddetectwithanti-AMP-activeproteinkinase(mouse,1:1,000).Secondaryantibody:anti-mouseIgG-HRP(1:10,000).Membrane:Hyond™P.Detection:Hyperfilm™ECL.Signalexposedfor30secondsanddetectedbyX-ray.SignalresultpresentbynormalECLsubstrateisstrongerthanusingexpiredECLsubstrate.LuminolPen™notedmarkercanbeanindicatortochecktheresultoradjustdifferentexperimenttosimilarexperimentalcondition.

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