VisualProtein/HybriMore™ Hybridoma Culture Supplement/1 bottle For 1 L culture medium dilution/HB01-1L

HybriMore™HybridomaCultureSupplement

Growthpromotingsupplementespeciallyforhybridomacells

HybriMore™Hybridomacloningfactorisaspecialsupplementwhichisaddinginthemediumwhenculturinghybridomacells.Itcansubstantiallyprovidenecessarygrowthfactorduringcellcultureandthereforesuccessfullyincreasethecloningefficiencyandraisethesurvivalrateofhybridomacell.Nonegativeeffecttohybridomacellwithdefinedchemicalcomponentanddefinedconcentration.

SpecialCharacteristic

• Increasecloningefficiencyandcellsurvivalrate
• Culturinghybridomacellswithbetterantibodysecretionandproduction
• Growthpromotingsupplementwithdefinedchemicalcomponentandconcentration

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Figure1.Comparisonofthecloningefficiencyofthenewlyfusedhybridomacells.ThenewlyPEGfusedhybridomacellswereplatedintoa96-wellplatecontainingFCSmediawithHybriMore™(greenbars),FCSmediawithfeederlayer(bluebars),orregularFCSmedia(whitebars).HybridomacellsweresubjecttoHATselection14daysafterthecellfusion.Thenumbersofviablehybridomacolonieswerevisuallycountedunderamicroscope.Twomousemyelomafusionpartners,NS-1andSP2/0,wereevaluatedbyfourindependentfusionexperimentswithfreshlypreparedmousespleens.

Figure2.Comparisonofsuccessfulrateofmonoclonizinghybridomacells.EightclonesofhybridomacellsfromNS-1orSP2/0fusionpartnersweremonoclonizedinthemediacontainingFCSmediawithHybriMore™(greenbars),FCSmediawithfeederlayer(bluebars),orregularFCSmedia(whitebars).Thenumbersofviablehybridomacoloniesinawellwerevisuallycountedunderamicroscope.

Figure3.ComparisonofthetitersofsecretingAb.Acloneofhybridomacells(antihumantransferrin,L3B5)wasculturedinthemediacontainingFCSmediawithHybriMore(greenbars),FCSmediawithfeederlayer(bluebars),orregularFCSmedia(whitebars)forsevendays.ThesupernatantswereharvestandexaminedbythetiterofsecretingAbbyELSIAassay.

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