VisPRO™5MinutesProteinStainKitutilizesimidazoleandzincionsprecipitateinthegelandrevealtheproteinpositioninonly5minutes.Thedetectionsensitivitycanbereachedto1ng,competitivewithsilverstainandSYPROruby.VisPRO™stainsthegelratherthantheproteinsampleandwillnotinteractwiththeproteinsample.Thestainedtargetproteincanbeeluteddirectlyandapplydownstreamexperiments,suchasWesternblotting,LCMSMSanalysis.Thestainingsolutionisalsohighlystable,non-biohazardtooperatorandenvironment.
Cat.No. | ProductName | Description |
VP01-125 | VisPRO™5MinuteProteinStainKit | 1kit125mlsolution1+125mlsolution2 |
VP01-500 | VisPRO™5MinuteProteinStainKit | 1kit500mlsolution1+500mlsolution2 |
VP05-125 | VisPRO™5MinuteProteinStainKit | 1kit5Xconcentratesolution125mlsolution1+125mlsolution2 |
VP05-500 | VisPRO™5MinuteProteinStainKit | 1kit5Xconcentratesolution 500mlsolution1+500mlsolution2 |
VP10-1L | VisPRO™5MinuteProteinStainKit | 1kit10Xconcentratesolution1,000mlsolution1+1,000mlsolution2 |
Figure1.Positivestainingvs.Reversestaining
Figure2.ComparisonthestainingtimebyusingVisPRO™5minutesProteinStainKitandotherstaining.
Figure3.Comparisonofstainingsensitivity.ThecommercialproteinMarkers(Amersham,GEhealthcare)wereusedforevaluation.Numbersshownabovethegelsindicatetheactualamountofbovineserumalbuminprotein(indicatedbyarrow).
Figure4.CompatibilityoftheVisPRO™5minutesProteinStainKitwithotherapplications.
Table1.Comparisonofstainingmethods.
MSDS
Manual
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn