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Hemoglobin Depletion and Protein Enrichment From Dried Blood Spots
Hemoglobin is a common contaminant from dried whole blood cards and not normally found in serum samples. The HemogloBind™ Blood Card protocol was designed to substantially reduce the presence of hemoglobin and its associated interference with many serum proteins and other analytes.
HemogloBind™ is engineered for a high degree of selectivity and does not cross react with most common serum components, making it an excellent sample prep in numerous applications. These include analytical protocols where optical interference is problematic, such as bilirubin and cholinesterase analysis.
| Dried Blood Spot (µl) | Hemoglobin Present (Based on 200mg Hb per ml blood) (mg) | Extraction Buffer (µl) | HemogloBind™ Reagent Used (ml) | Protein Recovery (µg) | Hemoglobin removal (%) |
|---|---|---|---|---|---|
| 20 | 4 | 200 | 200 | 400 - 500 | > 95% |
| 10 | 2 | 100 | 100 | 200 - 250 | > 95% |
| 5 | 1 | 50 | 50 | 100 - 125 | > 95% |
| 2.5 | 0.5 | 25 | 25 | 90 - 100 | > 95% |
| Product | # of samples processed | Item No. |
|---|---|---|
| HemogloBind™ Blood Card | 10 Dried Whole Blood Spot (7mm hole punch) | H0145BC-10 |
| HemogloBind™ Blood Card | 50 Dried Whole Blood Spot (7mm hole punch) | H0145BC-50 |
| Kit Content | 10 Prep | 50 Prep | Reagent |
|---|---|---|---|
| HemogloBind™ | 1 ml | 5 ml | Supplied |
| Protein Extraction Buffer (PEB) | 1 ml | 5 ml | Supplied |
| Spin-X Centrifuge tube filters | 10 | 50 | Supplied |
| Suggested Or Equivalent Supplier of Blood Card: Whatman 903™ Protein Saver cards | Not Supplied |
Click Here To View HemogloBind™ Blood Card Product Sheet
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