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Suspension Reagent to remove Hemoglobin interference for PCR
Some infectious viruses are known or suspected to be associated with red cells, such as Zika and West Nile viruses. So to detect their presence, it is becoming increasingly apparent to use whole blood, rather than plasma alone, as a starting sample type. However, the presence of hemoglobin from whole blood lysates is challenging for PCR because of the large amount of hemoglobin present. HemogloBind™ Nucleic Acid is designed to efficiently remove hemoglobin while maintaining very high recovery of nucleic acids, both RNA and DNA.
MATERIALS
Materials required | 5 ml | 50 ml |
HemogloBind™ Nucleic Acid suppliedas Suspension Reagent in 10 % Isopropanol | 5ml (25 prep) | 50ml (200 prep) |
Microfuge Spin-X type Filters | 25supplied | Recommended (Notsupplied) |
3M Guanidine ThioCyanate (orequivalent) | Notsupplied | Notsupplied |
Wide bore pipette tips | Suggested(Not Supplied) | |
HemogloBind™ Nucleic Acid 5ml (includes Spin filters) | H0250-05 |
HemogloBind™ Nucleic Acid 50ml (reagent only) | H0250-50 |
Click Here To View HemogloBind™ Product Sheet
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