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VitaNavi/Alpha Taq & Alpha Taq LA/VN176E

AlphaTaqTMisanewgenerationmutantofTaqpolymerase.ThemutationsinTaqpolymerasegenerendertheenzymeresistanttotheinhibitoryeffectsofhigherconcentrationsofblood,soil,plantandmore.Ittypicallyremainsfunctionalin20-30%wholebloodinPCR,andinsomeconcentrationsofcrudesoilextractswhereothercommercialenzymesfail.ItcantoleratemoreinthepresenceofPCRenhancercocktail.Itisabletoamplifythetargetgenedirectlyfromwholeblood,serum,plasma,water,milk,tissueandplantleaf,etc.withoutDNApurificationpriortoPCR.Thisenzymecanbeusedinreal-timePCRwithbothDNAbindingdyes,suchasSYBRGreenandEvaGreen,andTaqManassaywhichrequires5’-3’exonucleaseactivity.

AlphaTaqTMLA version(Long&Accurate)isavailable,whichallowsforamplificationoflongerproductswithhigherfidelityandaccuracy.

AlphaTaqTMandAlphaTaqTM LAMix are availableasaready-to-use2XmixtureofDNApolymerase,salts,magnesiumanddNTPsforsettingupatrouble-freePCRreactionandthereisthechoiceofaninertgreendye,usedfordirectgelloADIng.Allyouhavetodoistoaddtemplate,specificprimersandwater(Clickhere).

  • ThermostABIlity  morethermostablethanstandardTaqDNApolymerase
  • Efficient–novelbuffersystemmaximizesefficiencyofPCRamplification,increasesyieldofanyPCRproduct
  • RobustandInhibition-resistant–reliabledirectamplification fromcrudesamplesandwitheventhemostchallengingDNAtargets,especiallywhencombiningwithourpowerfulPCRenhancers
  • FlexIBLe–LAversionisidealforamplifyinganytargetupto30kbfromDNAextractedfromhuman,animalandplantsamples
DataSheet:AlphaTaq,AlphaTaqLA
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