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VitaNavi/2X ReadyGo PCR Master Mix/VN601MC

2XReadyGoPCRMasterMixisa2Xconcentratedsolutionof Taq DNAPolymerase,dNTPs,andallofthecomponentsrequiredforPCR,exceptDNAtemplateandprimers.Thispre-mixedformulationsavestimeandreducescontaminationduetoareducednumberofpipettingstepsrequiredforPCRsetup.ThemixisoptimizedforefficientandreproducIBLePCR.

TheMasterMixhasfourversions,withstandardbufferandGCbuffer,colorlessandgreen.TheMasterMixwithGCbufferisusedforamplificationofGC-richtarget(GCcontentis>65%).ThegreenoneissupplementedwithtwotrackingdyesfortheconvenienceofdirectgelloADIngofPCRproducts.

Ona1%agarosegelin1XTBE,onedyemigratesatapproximately4kbandanothermigratesatapproximately50bp.

Highlights

  • Convenient:Ready-to-usemix,directloadingofPCRproductsongel
  • Thermostable:Enzymeinthemixretainsatleast85%activityafter60minat95°C
  • GeneratesPCRproductswith3"-dAoverhangs
  • Incorporatesmodifiednucleotides (e.g.,biotin-,digoxigenin-,fluorescently-labelednucleotide)
  • Efficientamplificationofcomplextarget

DataSheet:2XReadyGoPCRMasterMix

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