NewEast/RhoA [26007][100 µL]/26007

Catalog Number:  26007

Gene Symbol:  RHOA

Description:   Anti-RhoA Mouse Monoclonal Antibody

Background:   Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca2+ sensitivity of smooth muscle contraction.

Immunogen:   : Recombinant full length protein of RhoA.

Applications:   ELISA, WB, IHC

Recommended Dilutions:   ELISA 1:1000-1:5000WB 1:1000-1:2000

Concentration:   1 mg/ml

Host:   mouse

Format:   Liquid

Clonality:   Monoclonal

Isotype:   IgG

Purity:   Purified from ascites

Storage buffer:

Preservative:  no

Constituents:  : PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity:  RhoA antibody recognizes RhoA from vertebrates.

Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Western Blot:

Western blot analysis of RhoA proteins. MEF whole-cell lysate was used for western blot with the anti-RhoA mouse monoclonal antibody (Cat. # 26008).

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26007	Anti-RhoA Mouse Monoclonal Antibody	100 μL	$239
21017	Anti-RhoA Rabbit Polyclonal Antibody	100 μL	$219

Publications:

1.  Ethanol increases p190RhoGAP activity, leading to actin cytoskeleton rearrangements    J Neurochem. 2011 Dec;119(6):1306-16

2.  The transcriptional coactivators megakaryoblastic leukemia 1/2 mediate the effects of loss of the tumor suppressor deleted in liver cancer 1    Oncogene. 2012 Aug 30;31(35):3913-23

3.  G(alpha)12/13 inhibition enhances the anticancer effect of bortezomib through PSMB5 downregulation    Carcinogenesis. 2010 Jul;31(7):1230-7

4.  Transgenesis of the Wolffian duct visualizes dynamic behavior of cells undergoing tubulogenesis in vivo    Development, Growth & Differentiation. Volume 55, Issue 4, pages 579–590, May 2013

5.  Foxc2 induces Wnt4 and Bmp4 expression during muscle regeneration and osteogenesis    Cell Death and Differentiation (2013) 20, 1031–1042

6.  N-glycosylation induces collagen triple helix repeat containing 1 (CTHRC1) and drives oral cancer cell migration    J Biol Chem. 2013 Jul 12;288(28):20217-27

7.  Cyclodextrin protects podocytes in diabetic kidney disease    Diabetes. 2013 Nov;62(11):3817-27

8.  Amyloid beta1-42 (Aβ42) up-regulates the expression of sortilin via the p75NTR/RhoA signaling pathway    Journal of NeurochemistryVolume 127, Issue 2, pages 152–162, October 2013

9.  Podocyte-specific GLUT4 deficient mice have fewer and larger podocytes and are protected from diabetic nephropathy    Diabetes. 2014 Feb;63(2):701-14

10.  Loss of TAK1 increases cell traction force in a ROS-dependent manner to drive epithelial–mesenchymal transition of cancer cells    Cell Death and Disease (2013) 4, e848

11.  Syndecan-2 regulation of morphology in breast carcinoma cells is dependent on RhoGTPases    Biochimica et Biophysica Acta (BBA) - General SubjectsVolume 1840, Issue 8, August 2014, Pages 2482–2490

12.  Epithelial repair is a two-stage process driven first by dying cells and then by their neighbours    J Cell Sci. 2014 Mar 15;127(Pt 6):1229-41

13.  MIF inhibits monocytic movement through a non-canonical receptor and disruption of temporal Rho GTPase activities in U-937 cells    CytokineVolume 69, Issue 1, September 2014, Pages 47–55

14.  Triclosan Potentiates Epithelial-To-Mesenchymal Transition in Anoikis-Resistant Human Lung Cancer Cells    PLoS One. 2014 Oct 16;9(10):e110851