Trichoplusia ni Cell Line (TniFNL) Kerafast

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Trichoplusia ni cell line derived from BTI-Tn-5B1-4, adapted for suspension growth and serum-free growth.

Highlights

Used for protein expression by baculovirus infection Can grow efficiently at 21 or 27 C in adherent or suspension conditions

The baculovirus expression vector system (BEVS) has proven to be a powerful technology for expression of recombinant proteins in eukaryotic cells. The BEVS technology has many advantages that include the capacity for large DNA insertions, the capacity for simultaneous expression of multiple genes, high expression levels for many proteins, and posttranslational modifications that often resemble those found in mammalian cells.

From the laboratory of Dominic Esposito, PhD, National Cancer Institute/NIH.

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Use of 10 units/ml of Heparin can be used on highly aggregated cultures to disperse aggregation
From the laboratory of Dominic Esposito, PhD, National Cancer Institute/NIH.
We generally start the culture in 20 ml supplemented with some serum (10% FBS) shaking in a 125 ml non baffled flask at 140 RPM for a 1” throw.
Mehalko JL, Esposito D. Engineering the transposition-based baculovirusexpression vector system for higher efficiency protein production from insectcells. J Biotechnol. 2016 Nov 20;238:1-8.Gillette WK, Esposito D, Abreu Blanco M, Alexander P, Bindu L, Bittner C,Chertov O, Frank PH, Grose C, Jones JE, Meng Z, Perkins S, Van Q, Ghirlando R,Fivash M, Nissley DV, McCormick F, Holderfield M, Stephen AG. Farnesylated andmethylated KRAS4b: high yield production of protein suitable for biophysicalstudies of prenylated protein-lipid interactions. Sci Rep. 2015 Nov 2;5:15916.Gillette W, Frank P, Perkins S, Drew M, Grose C, Esposito D. Production ofFarnesylated and Methylated Proteins in an Engineered Insect Cell System. MethodsMol Biol. 2019;2009:259-277. View Article

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