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Kerafast/Anti-ADAM17 (mouse), Cytoplasmic Domain Antibody/EHS008/100uL

ThisrabbitIgGpolyclonalantibodywasgeneratedagainsttheGST-ADAM17-cytoplasmictailandisspecificforthecytoplasmicdomainofmouseADAM17.

TheADAM(ADisintegrinAndMetalloproteaseDomain)familyisauniquegroupofproteinsthathastheABIlitytocleaveorshedextracellularportionsoftransmembraneproteins.Inaddition,ADAMsareinvolvedincell-cellandcell-matrixinteractions(e.g.,fertilization,muscledevelopmentandneurogenesis).

ADAM17(alsoreferredtoasTumorNecrosisFactorConvertase,TACE)isinvolvedintheprocessingoftumornecrosisfactoralpha(TNF-a)atthesurfaceofthecell,andisalsorequiredforsheddingseveralligandsoftheepidermalgrowthfactorreceptorandmanyothermembraneproteins.

FromthelaboratoryofCarlP.Blobel,MD,PhD,HospitalforSpecialSurgery.

The Investigator PartofTheInvestigator"sAnnexeprogram.

Specifications

ProductType:	Antibody
Antigen:	GST-ADAM17CytoplasmcDomain
MolecularWeight:	~100kDa(matureform),~120kDa(pro-form)
Isotype:	IgG
Clonality:	Polyclonal
Reactivity:	Mouse
Immunogen:	GST-cytocorrespondingtothecytoplasmicdomainofmouseADAM17
SpeciesImmunized:	Rabbit
Epitope:	Cytoplasmicdomain
PurificationMethod:	Serum,notpurified
TestedApplications:	WB(1:1000)
Storage:	-80C
Shipped:	Dryice

ADAM17(mouse),CytoplasmicDomainAntibody

AntigenName:	GST-ADAM17CytoplasmcDomain
MolecularWeight:	~100kDa(matureform),~120kDa(pro-form)
ImmunoglobulinIsotype:	IgG
Clonality:	Polyclonal
SpeciesReactivity:	Mouse
TypeofImmunogen:	GST-cytocorrespondingtothecytoplasmicdomainofmouseADAM17
Epitope:	Cytoplasmicdomain
SpeciesImmunized:	Rabbit
PurificationMethod:	Serum,notpurified
TestedApplications:	WB(1:1000)
StorageTemperature:	-80C
Shipped:	Dryice

FindthebestADAMAntibodyforyourresearch:

Antibody	SpeciesReactivity	Specificity
ADAM9	Human	CytoplasmicDomain
ADAM9	Mouse	CytoplasmicDomain
ADAM12	Mouse	CytoplasmicDomain
ADAM15	Human	CytoplasmicDomain
ADAM15	Mouse	CytoplasmicDomain
ADAM15	Human	ExtracellularDomain
ADAM15	Mouse	DisintegrinDomain
ADAM17	Mouse	CytoplasmicDomain
ADAM17	Mouse	Prodomain
ADAM19	Mouse	CytoplasmicDomain
ADAM28	Mouse	CytoplasmicDomain
ADAM28	Mouse	ExtracellularDomain
SNX9	Mouse	CytoplasmicDomain

Comments

Celllysisbuffermustcontain5mM1,10PhenanthrolinetopreventautodegradationofthematureformofADAM17,whichisnotpreventedbyEDTAortypicalproteaseinhibitorcocktails(Schlöndorffetal.,BiochemJ.2000,347,131-138).

Data

WesternBlotAnalysis

Antibodies:prodomainfusedtoGST(α-PRO),GST-fusionproteincontainingthecyotplasmicdomain(α-CYTO).

Adaptedfrom:SchlöndorffJ,etal.JBiochemJ.2000,347,131-138.

References

Schlöndorff,J.;Becherer,J.D.;Blobel,C.P.Intracellularmaturationandlocalizationofthetumournecrosisfactoralphaconvertase(TACE).BiochemJ.2000,347,131-138.
Zheng,Y.;Schlondorff,J.;Blobel,C.P.Evidenceforregulationofthetumornecrosisfactoralpha-convertase(TACE)byprotein-tyrosinephosphatasePTPH1.J.Biol.Chem.2002,277,42463-42470.
Horiuchi,K.;KimuraT.;MiyamotoT.;TakaishiH.;OkadaY.;Toyama,Y.,Blobel,C.P.Cuttingedge:TNF-alpha-convertingenzyme(TACE/ADAM17)inactivationinmousemyeloidcellspreventslethalityfromendotoxinshock.J.Immunol.2007,179,2686-2689.
LeGall,S.M.,Maretzky,T.,Issuree,P.D.,Niu,X.D.;Reiss,K.,Saftig,P.,Khokha,R.,Lundell,D.,Blobel,C.P.ADAM17isregulatedbyarapidandreversIBLemechanismthatcontrolsaccesstoitscatalyticsite.J.Cell.Sci.2010,123,3913-3922.
Maretzky,T.,Evers,A.,Zhou,W.,Swendeman,S.L.,Wong,P.M.,Rafii,S.,Reiss,K.,BlobelC.P.Migrationofgrowthfactor-stimulatedepithelialandendothelialcellsdependsonEGFRtransactivationbyADAM17.Nat.Commun.2011,2,1-25.

Ifyoupublishresearchwiththisproduct,pleaseletusknowsowecanciteyourpaper.

Research

Antibody	SpeciesReactivity	Specificity
ADAM9	Human	CytoplasmicDomain
ADAM9	Mouse	CytoplasmicDomain
ADAM12	Mouse	CytoplasmicDomain
ADAM15	Human	CytoplasmicDomain
ADAM15	Mouse	CytoplasmicDomain
ADAM15	Human	ExtracellularDomain
ADAM15	Mouse	DisintegrinDomain
ADAM17	Mouse	CytoplasmicDomain
ADAM17	Mouse	Prodomain
ADAM19	Mouse	CytoplasmicDomain
ADAM28	Mouse	CytoplasmicDomain
ADAM28	Mouse	ExtracellularDomain
SNX9	Mouse	CytoplasmicDomain

Data

WesternBlotAnalysis

Antibodiesagainst:pro-domainfusedtoGST(α-PRO),cytoplasmicdomainfusedtoGST(α-CYTO).

Adaptedfrom:SchlöndorffJ,etal.JBiochemJ.2000,347,131-138.

Provider

FromthelaboratoryofCarlP.Blobel,MD,PhD,HospitalforSpecialSurgery.

Comments

References

Schlöndorff,J.;Becherer,J.D.;Blobel,C.P.Intracellularmaturationandlocalizationofthetumournecrosisfactoralphaconvertase(TACE).BiochemJ.2000,347,131-138.
Zheng,Y.;Schlondorff,J.;Blobel,C.P.Evidenceforregulationofthetumornecrosisfactoralpha-convertase(TACE)byprotein-tyrosinephosphatasePTPH1.J.Biol.Chem.2002,277,42463-42470.
Horiuchi,K.;KimuraT.;MiyamotoT.;TakaishiH.;OkadaY.;Toyama,Y.,Blobel,C.P.Cuttingedge:TNF-alpha-convertingenzyme(TACE/ADAM17)inactivationinmousemyeloidcellspreventslethalityfromendotoxinshock.J.Immunol.2007,179,2686-2689.
LeGall,S.M.,Maretzky,T.,Issuree,P.D.,Niu,X.D.;Reiss,K.,Saftig,P.,Khokha,R.,Lundell,D.,Blobel,C.P.ADAM17isregulatedbyarapidandreversiblemechanismthatcontrolsaccesstoitscatalyticsite.J.Cell.Sci.2010,123,3913-3922.
Maretzky,T.,Evers,A.,Zhou,W.,Swendeman,S.L.,Wong,P.M.,Rafii,S.,Reiss,K.,BlobelC.P.Migrationofgrowthfactor-stimulatedepithelialandendothelialcellsdependsonEGFRtransactivationbyADAM17.Nat.Commun.2011,2,1-25.
McIlwainDR,LangPA,MaretzkyT,HamadaK,OhishiK,ManeySK,BergerT,MurthyA,DuncanG,XuHC,LangKS,HäussingerD,WakehamA,Itie-YoutenA,KhokhaR,OhashiPS,BlobelCP,MakTW.iRhom2regulationofTACEcontrolsTNF-mediatedprotectionagainstListeriaandresponsestoLPS.Science.2012Jan13;335(6065):229-32.

Ifyoupublishresearchwiththisproduct,pleaseletusknowsowecanciteyourpaper.

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Kerafast/Anti-ADAM17 (mouse), Cytoplasmic Domain Antibody/EHS008/100uL

Comments

Data

References