产品资料

Kerafast/Anti-Actin-1 [mAb45a] Antibody/EGA002/100ug

ThismousemonoclonalantibodywasgeneratedagainstplantactinACT1andrecognizessubclasses4and5ofvegetativeplantactins(arABIdopsisACT1,3,4,12).

ActinexistsasaubiquitousproteininvolvedwithfilamentformationthatmakeuplargeportionsoftheCytoskeleton.Actinfilamentsinteractwithmyosintoassistinmusclecontractionaswellasaidingincellmotilityandcytokinesis.Invertebratestherearethreegroupsofactinisoforms:alpha,betaandgamma.Thealphaactinsarefoundinmuscletissuesandareamajorconstituentofthecontractileapparatus.Thebetaandgammaactinsco-existsinmostcelltypesascomponentsofthecytoskeletonandasmediatorsofinternalcellmotility.

FromthelaboratoryofRichardB.Meagher,PhD,UniversityofGeorgia.

The Investigator PartofTheInvestigator"sAnnexeprogram.

Specifications

ProductType:	Antibody
AccessionID:	AEC09427
Antigen:	ArabidopsisActin(ACT1)
MolecularWeight:	45kDa
Isotype:	IgG1
Clonality:	Monoclonal
CloneName:	mAb45a(5-15H7C5)
Reactivity:	Plantactins
Immunogen:	PurifiedArabidopsisactin(ACT1)
SpeciesImmunized:	Mouse
Epitope:	Asn79inreproductiveplantactins
PurificationMethod:	ProteinGcolumn
Buffer:	0.1MSodiumPhosphate,pH7.4,0.15MNaCl,0.05%(w/v)SodiumAzide
TestedApplications:	WB(1-2ug/mL)
Storage:	-20C
Shipped:	Coldpacks

Research

Antibody	Reactivity
Actin[mAbGEa]	Universal
Actin-1[mAb45a]	ACT1,3,4,12
Actin-11[mAb2345a]	ACT1,3,4,7,11,12
Actin-2[mAb13a]	ACT2,8,11
Actin-8[mAbGPa]	ACT1,2,3,4,7,8,11,12

Data

WesternBlotAnalysis

Adaptedfrom:Kandasamy,M.K.,etal.(2001)PlantCell13,1541-54.

Provider

FromthelaboratoryofRichardB.Meagher,PhD,UniversityofGeorgia.

References

Kandasamy,M.K.,Gilliland,L.U.,McKinney,E.C.,andMeagher,R.B.(2001).Oneplantactinisovariant,ACT7,isinducedbyauxinandrequiredfornormalcallusformation.PlantCell13,1541-54.
Kandasamy,M.K.,McKinney,E.C.,andMeagher,R.B.(1999).Thelatepollen-specificactinsinangiosperms.PlantJ18,681-91.
Kandasamy,M.K.,McKinney,E.C.,Roy,E.,andMeagher,R.B.(2012).Plantvegetativeandanimalcytoplasmicactinssharefunctionalcompetenceforspatialdevelopmentwithprotists.PlantCell24,2041-57.

Ifyoupublishresearchwiththisproduct,pleaseletusknowsowecanciteyourpaper.

AntigenName:	ArabidopsisActin(ACT1)
AccessionID:	AEC09427
MolecularWeight:	45kDa
ImmunoglobulinIsotype:	IgG1
Clonality:	Monoclonal
CloneName:	mAb45a(5-15H7C5)
SpeciesReactivity:	Plantactins
TypeofImmunogen:	PurifiedArabidopsisactin(ACT1)
Epitope:	Asn79inreproductiveplantactins
SpeciesImmunized:	Mouse
PurificationMethod:	ProteinGcolumn
StorageBuffer:	0.1MSodiumPhosphate,pH7.4,0.15MNaCl,0.05%(w/v)SodiumAzide
TestedApplications:	WB(1-2ug/mL)
StorageTemperature:	-20C
Shipped:	Coldpacks

对照品标准品，生物试剂盒，gibco胎牛血清，试剂采购网，中国试剂网，细胞因子试剂盒

Kerafast/Anti-Actin-1 [mAb45a] Antibody/EGA002/100ug

Data

References