- Specifications
- Tech Info / Data
- Citations
- Documentation
Specifications
Compatibility: Tissue Culture MediaELISA Assay Range: 1.95 - 125 pg/mlAssay Length: 20 hours 30 minutes - 24 hours 30 minutesSpecificity: Human Interferon AlphaCross-reacts with Cynomolgus/Rhesus IFN-α (13%). No cross-reactivity against human IFN-β, IFN-γ, or IFN-ω; mouse or rat IFN-α, IFN-β or IFN-γ; pig IFN-α; or bovine IFN-τ.
Inter-Assay CV: ≤ 10%Intra-Assay CV: ≤ 8%Average Spike Recovery: 105%
Storage: 2-8°CExpiration Date: One year from date of manufactureShipping Condition: Wet Ice
Materials Provided:
- Pre-coated microtiter plate
- Plate Sealers
- Wash Solution Concentrate
- Human Interferon Alpha Standard, 10,000 pg/ml
- Assay Buffer
- Standard Diluent
- Antibody Diluent
- Antibody Concentrate
- HRP Conjugate Concentrate
- HRP Diluent
- TMB Substrate Solution
- Stop Solution
Tech Info / Data
Tips, Tools and Troubleshooting:
- Human IFN-α ELISA Subtype Specificities Comparison Chart
Detailed Related Product Information:
- Quantitative Immunoassays: Single Analyte Screening Services
PBL’s VeriKine-HS Human Interferon Alpha All Subtype TCM ELISA Kit is specifically formulated to detect all human IFN-Alpha subtypes. This kit quantitates all human IFN alpha subtypes in tissue culture media (TCM) using a sandwich immunoassay format. The kit is based on an ELISA with biotinylated-detection antibody and streptavidin-conjugated horseradish peroxidase (HRP). Tetramethyl-benzidine (TMB) is the substrate. The assay is based on the international reference standard for human interferon alpha provided by the National Institutes of Health.
Interferons (IFNs) are a family of mammalian cytokines initially characterized by their ability to inhibit viral infection. They are synthesized and secreted by most cell types in response to pathogens. In addition to their antiviral properties, IFNs have also been shown to exhibit anti-proliferative, immunomodulatory, and many other activities.
In humans, IFN-α refers to a family of proteins comprised of 12 highly homologous protein subtypes (greater than 85% by amino acid sequence), encoded by 13 genes, that exhibit pleiotropic biologic activities. Measuring the levels of the 12 different human IFN-α subtypes is essential for understanding their triggers and subsequent effects on the immune system. The ability to measure all the subtypes may provide an indication of the total IFN-α level.
Citations
Documentation
41135 Certificate of Analysis & Protocol (One-Page) 41135 Technical Data Sheet 41135 Protocol (Full) 41135 Certificate of Analysis (CoA) Safety Data Sheet (SDS) 41135 FlyerTypical Standard Curves in Various Matrices using PBL"s High Sensitivity Human IFN Alpha TCM ELISA (41135)
