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Description
Details
BioLite™ surface markers allow for direct immunofluorescent staining of live, unfixed pluripotent stem cell populations. The antibodies undergo meticulous application testing and validation to ensure they are sterile, non-toxic, contain no sodium azide, and are free of culture pathogens. Colonies stained with BioLite antibodies are able to continue in culture with no adverse effect on proliferation and differentiation potential when compared to untreated cells.Background Stage-specific embryonic antigen-1 (SSEA-1) is a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma (EC), murine embryonic stem (ES), and murine and human germ (EG) cells. No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. SSEA-1 is associated with cell adhesion, migration and differentiation.
Details
Details
| Concentration | 0.5 mg/mL |
|---|---|
| Species Reactivity | Human, Mouse |
| Host | Mouse Monoclonal |
| Clone | MC-480 |
| Isotype | IgM, κ |
| Immunogen | F9 tetracarcinoma stem cells (X-irradiated) |
| Formulation | 0.2 μm filter sterilized phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 eu/μg="" of="" the="" protein=""><0.01 ng/μg="" of="" the="" protein)="" as="" determined="" by="" the="" lal="" test.="" formulation="" is="" free="" from="" bacteria,="" fungi,="" and=""> |
| Storage and Stability | Store at 2-8°C protected from light. Stable for 6 months from date of receipt when stored as directed. The BioLite format contains no preservative and therefore must be handled under aseptic conditions. |
| Applications Tested | Immunofluorescence (IF) on live, unfixed mouse ES cells |
| Recommended Dilutions | Immunofluorescence 1:100 Flow Cytometry 1:100 It is recommended that the antibody be titrated for optimal performance for each application. |
| Alternative Names | CD15, Stage-specific embryonic antigen 1 |
| References | Brambrink, T., et al. (2008) Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell 2: 151-159. PMID: 18371436Draper, J.S., et al. (2002) Surface antigens of human embryonic stem cells: changes upon differentiation in culture. J Anat 200: 249-258. PMID: 12033729 |
| Technical Documents | ST11006 Technical Data Sheet Protocol: Immunofluorescent Staining of Live Cells |