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This antibody recognizes the stage-specific embryonic antigen-3 (SSEA-3) that is expressed upon the surface of human teratocarcinoma stem (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells. No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-3 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES/iPS cells may be accompanied by an increase in SSEA-3 expression.
Sample Data
Figure A: FC analysis on H1 human ES cells at a 1:100 dilution.Red histogram represents SSEA-3 Antibody and open histogram represents isotype control. A PE-conjugated anti-Rat IgM was used as the secondary antibody. Figure B: ICC analysis on H1 human ES cells. Cells were stained with SSEA-3 Antibody at a 1:100 dilution followed by an Alexa 488-conjugated secondary antibody (green). Nuclei were counterstained with DAPI (blue).
| Concentration | 0.5 mg/mL |
|---|---|
| Species Reactivity | Human, Mouse |
| Host | Rat Monoclonal |
| Clone | MC-631 |
| Isotype | IgM |
| Immunogen | 4-8 cell stage mouse embryos |
| Formulation | Aqueous buffer, 0.09% sodium azide. |
| Storage and Stability | Store at 2-8°C. Stable for 6 months from date of receipt when stored as directed. |
| Applications Tested | Flow Cytometry (FC), Immunocytochemistry/Immunofluorescence (ICC/IF) |
| Recommended Dilutions | Flow Cytometry 1:100Immunocytochemistry/Immunofluorescence 1:100It is recommended that the antibody be titrated for optimal performance for each application. |
| Alternative Names | Stage-specific embryonic antigen 3 |
| References | Brimble, S.N., et al. (2007) The cell surface glycosphingolipids SSEA-3 and SSEA-4 are not essential for human ESC pluripotency. Stem Cells 25: 54-62. PMID: 17008424Henderson, J.K., et al. (2002) Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells 20: 329-337. PMID: 12110702Kannagi, R., et al. (1983) Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells. EMBO J 2: 2355-2361. PMID: 6141938 |
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