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ESIBIO/SSEA-1 anti-Human/Mouse Antibody, 100 µL/100 µL/ST11013

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Description

Details

Stage-specific embryonic antigen-1 (SSEA-1) is a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma (EC), murine embryonic stem (ES), and murine and human germ (EG) cells. No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. SSEA-1 is associated with cell adhesion, migration and differentiation.

Sample Data antibody icc analysis Figure A: FC analysis of SSEA-1 Antibody on mouse ES cells at a 1:100 dilution. Green histogram represents SSEA-1 Antibody and open histogram represents isotype control. A PE-conjugated anti-Mouse IgM was used as the secondary antibody. Figure B: ICC analysis on mouse ES cells. Cells were stained with SSEA-1 Antibody at a 1:100 dilution followed by an Alexa Fluor 594-conjugated secondary antibody (red). Nuclei were counterstained with DAPI (blue).

Details

Details

Concentration0.5 mg/mL
Species ReactivityHuman, Mouse
HostMouse Monoclonal
CloneMC-480
IsotypeIgM
ImmunogenF9 tetracarcinoma stem cells (X-irradiated)
FormulationAqueous buffer, 0.09% sodium azide.
Storage and StabilityStore at 2-8°C. Stable for 6 months from date of receipt when stored as directed.
Applications Tested Flow Cytometry (FC), Immunocytochemistry/Immunofluorescence (ICC/IF)
Recommended DilutionsFlow Cytometry 1:100Immunocytochemistry/Immunofluorescence 1:100It is recommended that the antibody be titrated for optimal performance for each application.
Alternative NamesCD15, Stage-specific embryonic antigen 1
ReferencesBrambrink, T., et al. (2008) Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell 2: 151-159. PMID: 18371436Draper, J.S., et al. (2002) Surface antigens of human embryonic stem cells: changes upon differentiation in culture. J Anat 200: 249-258. PMID: 12033729
Technical Documents ST11013 Technical Data Sheet

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  • mouse embryonic stem cell (1)
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  • germ cell (1)
  • ICC (5)
  • mES (2)
  • flow (5)
  • IgM (2)

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