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Stage-specific embryonic antigen-1 (SSEA-1) is a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma (EC), murine embryonic stem (ES), and murine and human germ (EG) cells. No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. SSEA-1 is associated with cell adhesion, migration and differentiation.
Sample Data 
Figure A: FC analysis of SSEA-1 Antibody on mouse ES cells at a 1:100 dilution. Green histogram represents SSEA-1 Antibody and open histogram represents isotype control. A PE-conjugated anti-Mouse IgM was used as the secondary antibody. Figure B: ICC analysis on mouse ES cells. Cells were stained with SSEA-1 Antibody at a 1:100 dilution followed by an Alexa Fluor 594-conjugated secondary antibody (red). Nuclei were counterstained with DAPI (blue).
| Concentration | 0.5 mg/mL |
|---|---|
| Species Reactivity | Human, Mouse |
| Host | Mouse Monoclonal |
| Clone | MC-480 |
| Isotype | IgM |
| Immunogen | F9 tetracarcinoma stem cells (X-irradiated) |
| Formulation | Aqueous buffer, 0.09% sodium azide. |
| Storage and Stability | Store at 2-8°C. Stable for 6 months from date of receipt when stored as directed. |
| Applications Tested | Flow Cytometry (FC), Immunocytochemistry/Immunofluorescence (ICC/IF) |
| Recommended Dilutions | Flow Cytometry 1:100Immunocytochemistry/Immunofluorescence 1:100It is recommended that the antibody be titrated for optimal performance for each application. |
| Alternative Names | CD15, Stage-specific embryonic antigen 1 |
| References | Brambrink, T., et al. (2008) Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell 2: 151-159. PMID: 18371436Draper, J.S., et al. (2002) Surface antigens of human embryonic stem cells: changes upon differentiation in culture. J Anat 200: 249-258. PMID: 12033729 |
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