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GloboZymes Anti-AK/PAK2 is an affinity-purified polyclonal antibody produced in rabbits to a synthetic peptide corresponding to residues 273-291 of the catalytic domain of human lymphocyte PAK2. The antibody is useful for Western blotting. For example, it can be used at 1:1000 dilution with 20 μg HEK293 cell extracts. The antibody reacts with PAK2 and its catalytic domain from diverse tissue sources.
Synonyms: Polyclonal Antibody to Auto-Activated Protein Kinase; Autophosphorylation-Activated Protein Kinase; Catalytic Domain of PAK2; Catalytic Domain of p21-Activated Protein Kinase.
Background: The catalytic domain of PAK2 (AK/PAK2) was originally isolated as an auto-activated protein kinase (AK). Thus, the purified enzyme underwent rapid autophosphorylation and concomitant 15-30-fold activation. Subsequent discovery of the PAK family of enzymes and amino sequencing of AK revealed that AK was equivalent to the catalytic domain of PAK2. Unlike native PAK2, AK does not require p21-GTPases Cdc42 and Rac for expression of its activity. Interestingly, AK is a physiologically relevant form of PAK2. It is produced from the native enzyme via a caspase-dependent pathway in cells undergoing apoptosis.
Figure: Purified preparations of AK/PAK2 were subjected to Western Blot with GloboZymes affinity-purified rabbit polyclonal antibody Anti-AK/PAK2. The band correspond to AK/PAK2 (~ 36 kDa).
References: Proc Natl Acad Sci USA 90: 2500; J Biol Chem 268: 11193; J Cell Physiol 178: 397; J Biol Chem 276: 14909
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