产品资料

QuantaBio/sparQ PureMag Beads/60 mL/95196-060

Features & Benefits

High recovery of DNA fragments greater than 100 bp
Efficient removal of unwanted components from adapter ligation and PCR reactions
Consistent single or double-sided size selection
Seamless integration into existing NGS workflows with little or no protocol change
Easy-to-use and compatible with manual processing or automated liquid handling robots
Cost effective alternative to AMPure^® XP with equivalent performance

Description

sparQ PureMag Beads is a fast and reliable nucleic acid purification system for reaction cleanup and size selection in Next Generation Sequencing (NGS) workflows. Based on the reversible nucleic acid-binding properties of magnetic beads, this product can be used to quickly remove primers, primer-dimers, unincorporated nucleotides, salts, adapters and adapter-dimers from NGS library prep reactions to improve downstream sequencing performance. sparQ PureMag Beads allows excellent recovery of fragments greater than 100 bp without centrifugation or filtration. Consistent and reliable size selection can be achieved by simply adjusting the beads to sample ratio. This product is designed for both manual and automated processing, allowing seamless integration into existing workflows.

Workflow for Double-Sided Size Selection

Double-sided size selection tunable for specific application

Achieve consistent size range by simply manipulating the beads to sample ratio

Performance Data
Consistent and tunable size selection
Bioanalyzer trace of fragmented human genomic DNA pre- and post double-sided size selection
Bioanalyzer trace of fragmented human genomic DNA pre- and post double-sided size selection
Electropherogram of fragmented human genomic DNA pre- and post double-sided size selection. Different sparQ PureMag Beads to DNA ratios were used to achieve various targeted size range.
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Highly reproducible purification across a range of inputs
Highly reproducible purification across a range of inputs
Highly reproducible purification across a range of inputs
Highly reproducible DNA library profiles were achieved using different lots of sparQ PureMag Beads and a broad range of input amount. Libraries were prepared with sparQ DNA Library Prep Kit from 100 ng and 1 ng of fragmented microbial genomic DNA. sparQ PureMag Beads were used post adapter ligation and PCR amplification to effectively remove adapter-dimers and primer-dimers.
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Efficient recovery of DNA equivalent to AMPure XP
Efficient recovery of DNA equivalent to AMPure XP
Efficient recovery of DNA equivalent to AMPure XP
sparQ PureMag Beads show equivalent performance toAMPure XP for DNA purification. 50 bp DNA ladder was purified with sparQ PureMag Beads and AMPure XP at different beads to DNA ratios and analyzed on 2% agarose gel.
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QuantaBio/sparQ PureMag Beads/60 mL/95196-060

Features & Benefits

Description

Workflow for Double-Sided Size Selection

Workflow for Double-Sided Size Selection

Workflow for Double-Sided Size Selection

Performance Data

Bioanalyzer trace of fragmented human genomic DNA pre- and post double-sided size selection

Bioanalyzer trace of fragmented human genomic DNA pre- and post double-sided size selection

Highly reproducible purification across a range of inputs

Highly reproducible purification across a range of inputs

Efficient recovery of DNA equivalent to AMPure XP

Efficient recovery of DNA equivalent to AMPure XP

Performance Data

Consistent and tunable size selection

sparQ PureMag beads workflow

Consistent and tunable size selection

Bioanalyzer trace of fragmented human genomic DNA prior and post double-sided size selection

Highly reproducible purification across a range of inputs

Highly reproducible purification across a range of inputs

Efficient recovery of DNA equivalent to AMPure XP

Efficient recovery of DNA equivalent to AMPure XP