Bulk Adalimumab Biosimilar – Research Grade – Fc-Silenced
Product Benefits:
This biosimilar is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.
Target:
TNF alpha
Clone:
D2E7
Isotype:
Human IgG1 κ
Host:
Human
Species Reactivity:
Human
Specificity:
This non-therapeutic biosimilar antibody uses the same variable region sequence as the therapeutic antibody Adalimumab. Clone D2E7 binds to soluble TNF- α, but not to TNF- β (lymphotoxin).
Antigen Distribution:
TNF-α is secreted by macrophages, monocytes, neutrophils, T cells, B cells, NK cells, LAK cells.
Background:
Adalimumab is a research-grade monoclonal antibody that works by inactivating tumor necrosis factor-alpha (TNF-α). TNF-α is a 17.5 kD protein that mediates inflammation and immunity caused by the invasion of viruses, bacteria, and parasites by initiating a cascade of cytokines that increase vascular permeability, thus bringing macrophages and neutrophils to the site of infection. TNF-α secreted by the macrophage causes the blood to clot which provides containment of the infection. TNF-α inactivation has proven to be important in downregulating the inflammatory reactions associated with autoimmune diseases such as rheumatoid arthritis, psoriatic arthritis, ankylosing spondylitis, Crohn’s disease, moderate to severe chronic psoriasis, and juvenile idiopathic arthritis. Adalimumab blocks the interaction with the p55 and p75 cell surface TNF receptors thus, neutralizing the biological function of TNF. Anti-Human TNF alpha (Adalimumab) utilizes the same variable regions from the therapeutic antibody Adalimumab making it ideal for research projects.
Immunogen:
Human TNF alpha
Concentration:
≥ 2.0 mg/ml
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 – 7.4, 150 mM NaCl with no carrier protein, potassium, calcium or preservatives added.
Purity:
>99% by SDS-PAGE and HPLC
Endotoxin:
<0.5 EU/mg as determined by the LAL method
Storage:
This biosimilar is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Applications:
Blocking, ELISA, Functional Assays, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Neutralisation
Application Notes:
Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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