Bulk anti-CD127 In Vivo Antibody – Low Endotoxin (A7R34)
Product Benefits:
ichorbio’s anti-CD127 In Vivo Antibody – Low Endotoxin (A7R34) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.
Target:
CD127
Clone:
A7R34
Isotype:
Rat IgG2a
Other Names:
Interleukin-7 receptor subunit alpha, Il7r, IL-7R subunit alpha, IL-7R-alpha, IL-7RA
Uniprot:
P16872
Host:
Rat
Species Reactivity:
Mouse
Specificity:
Anti-CD127 In Vivo Antibody – Low Endotoxin (A7R34) recognizes an epitope on Mouse CD127
Purification Method:
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
IL-7 receptor alpha (IL-7Ra), also known as CD127, is expressed on bone marrow lymphoid precursors, pro-B cells, monocytes/macrophages, natural killer (NK) cells, naïve T cells and memory CD4+ and CD8+ T cells. IL-7Ra is also expressed on non-immune somatic cells such as intestinal colorectal and renal carcinoma cell lines and keratinocytes. IL-7Ralpha displays a synergistic decrease in the number of T-cell, NK-cell and multipotent progenitors in the thymus, indicating that these distinct survival pathways converge to promote the development of multipotent lymphoid progenitors.
Immunogen:
IL-7Ra-IgG1 fusion protein
Concentration:
1.0 mg/ml
Formulation:
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
Purity:
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
Endotoxin:
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation:
Aggregation level ≤ 5%
Aggregation level ≤ 1%
Storage:
anti-CD127 In Vivo Antibody – Low Endotoxin (A7R34) is stable when stored sterile at 2-8°C. Do not freeze.
Applications:
Immunoprecipitation, Western Blot, Blocking, Functional Assays, Flow Cytometry, IHC (Frozen), Immunofluorescence
Application Notes:
Blocking: Clone A7R34 is able to block clone SB/199 binding to IL-7R.Each investigator should determine their own optimal working dilution for specific applications.
Use:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Isotype Control:
Rat IgG2a In Vivo Isotype Control – Low Endotoxin [1-1] (ICH2244)
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