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immusmol/3 Catecholamines ELISA kit - Any sample - Research/BA-E-5600188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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immusmol/3 Catecholamines ELISA kit - Any sample - Research/BA-E-5600

Product informationKey stepsReferencesTechnical supportReviews

Product overview

Product name

Epinephrine-Norepinephrine ELISA kit - Research

DescriptionEnzyme immunoassay (ELISA) for the ultra-sensitive determination of epinephrine and norepinephrine in biological samples. Research use only.
Format3 x 96-well plate
SamplesAny sample
Sample volume1 - 750 µl
Cross specie-reactivityReact with all species
Standard rangeEpinephrine, Dopamine: 0 / 0.5 - 80 ng/ml; Norepinephrine: 0 / 0.2 - 32 ng/ml
Sensitivity

Epinephrine, Dopamine: 10 pg/ml Norepinephrine: 4 pg/ml

SpecificityNo significant cross-reactivity was observed with Epinephrine, Norepinephrine and Dopamine analogs such as Metanephrine, Normetanephrine, 3-Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid.
Assay timeSample preparation 4 hours and ELISA overnight
StorageStore at 2-8°C for to 6 months.
DatasheetsInstructions for use , Material safety datasheet
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Key steps

Sample preparation

Sample extraction and acylation (1h45), enzymatic conversion (2h)

ELISADopamine, Epinephrine, Norepinephrine antisera overnight incubation, revelation and read steps (1h).
Detailed protocolDownload instructions for use
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References

  • Horckmans M et al. Gene deletion of P2Y4 receptor lowers exercise capacity and reduces myocardial hypertrophy with swimming exercise. Am J Physiol Heart Circ Physiol. 2012 Oct 1;303(7):H835-43.
  • Schneeberger M et al. Deletion of miRNA processing enzyme Dicer in POMC-expressing cells leads to pituitary dysfunction, neurodegeneration and development of obesity. Molecular Metabolism. 2013 Apr. 
  • Ohlsson C et al. Estrogen receptor-α expression in neuronal cells affects bone mass. Proc Natl Acad Sci U S A. 2012 Jan 17;109(3):983-8.
  • Vida G et al. α7-cholinergic receptor mediates vagal induction of splenic norepinephrine. J Immunol. 2011 Apr 1;186(7):4340-6.
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Technical SupportSubmit a question

No technical question yet. Be the first to ask!Top

ReviewsSubmit your review

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