产品资料

immusmol/Dopamine ELISA kit - Research/BA-E-5300

Product overview

Product name	Dopamine ELISA kit - Research
Description	Enzyme immunoassay (ELISA) for the ultra-sensitive determination of Dopamine in biological samples. Research use only.
Format	96-well plate
Samples	Any sample
Sample volume	1 - 750 µl
Cross specie-reactivity	React with all species
Standard range	0 / 0.5 - 80 ng/ml
Sensitivity	3.3 pg/ml
Specificity	No significant cross-reactivity was observed with Dopamine analogs such as Adrenaline, Noradrenaline, Metanephrine, Normetanephrine, Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid.
Assay time	Sample preparation 4 hours and ELISA overnight
Storage	Store at 2-8°C for to 6 months.
Datasheets	Instructions for use , Material safety datasheet

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Key steps

Sample preparation	Sample extraction and acylation (1h45), enzymatic conversion (2h)
ELISA	Dopamine antiserum overnight incubation, revelation and read steps (1h).
Detailed protocol	Download instructions for use

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References

Product citations

Golomidov et al. The neuroprotective effect of fullerenols on a model of Parkinson’s disease in Drosophila melanogaster. Biochem Biophys Res Commun. 2019 Dec 23

Sabti et al. Elucidation of the Molecular Mechanism Underlying Lippia citriodora(Lim.)-Induced Relaxation and Anti-Depression. Int J Mol Sci. 2019 Jul 20

Robson MJ et al. σ Receptor antagonist attenuation of methamphetamine-induced neurotoxicity is correlated to body temperature modulation. Pharmacol Rep. 2013;65(2):343-9.
Seminerio MJ et al. The evaluation of AZ66, an optimized sigma receptor antagonist, against methamphetamine-induced dopaminergic neurotoxicity and memory impairment in mice. Int J Neuropsychopharmacol. 2013 Jun;16(5):1033-44.
Kim HG et al. Cyperi Rhizoma inhibits the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced reduction in nigrostriatal dopaminergenic neurons in estrogen-deprived mice. J Ethnopharmacol. 2013 Jun 21;148(1):322-8.
Kaushal N, Seminerio MJ, Robson MJ, McCurdy CR, Matsumoto RR. Pharmacological evaluation of SN79, a sigma (σ) receptor ligand, against methamphetamine-induced neurotoxicity in vivo. Eur Neuropsychopharmacol. 2013 Aug;23(8):960-71.
Zhang Z, Alexanian AR. Dopaminergic-like cells from epigenetically reprogrammed mesenchymal stem cells. J Cell Mol Med. 2012 Nov;16(11):2708-14.
Tasset I et al. Neuroprotective effects of extremely low-frequency electromagnetic fields on a Huntington"s disease rat model: effects on neurotrophic factors and neuronal density. Neuroscience. 2012 May 3;209:54-63.
Rönnberg E, Calounova G, Pejler G. Mast cells express tyrosine hydroxylase and store dopamine in a serglycin-dependent manner. Biol Chem. 2012 Jan;393(1-2):107-12.
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