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| AlternativeName: | PKB,ProteinkinaseB,RAC |
| Host: | Rabbit |
| Immunogen: | SyntheticpeptidecorrespondingtoaportionofhumanAkt1phosphorylatedatSer473. |
| UniProtID: | P31749 |
| GenBankID: | M63167 |
| Speciesreactivity: | Mouse |
| Crossreactivity: | Weaklycross-reactswithhuman,rat,bovineandchickenAkt. |
| Applications: | WB |
| RecommendedDilutions/Conditions: | WesternBlot(1µg/ml,ECL) Suggesteddilutions/conditionsmaynotbeavailableforallapplications. Optimalconditionsmustbedeterminedindividuallyforeachapplication. |
| ApplicationNotes: | Detectsabandof~60kDabyWesternblot. |
| PurityDetail: | Peptideaffinitypurified. |
| Formulation: | Liquid.InPBS,pH7.2,containing50%glyceroland0.09%sodiumazide. |
| Handling: | Avoidfreeze/thawcycles. |
| Shipping: | ShippedonBlueIce |
| LongTermStorage: | -20°C |
| ScientificBackground: | TheAkt(PKB)familyofproteinkinasesareserine/threoninekinases,withthreemammalianfamilymembersidentifiedtodate(Akt1,Akt2,Akt3).Aktisawell-characterizedmemberofPI3kinase-mediatedsignalingpathways,regulatingcellgrowth,apoptosis,glycogensynthesis,andothercellularresponsesthroughitsphosphorylationofdownstreamsubstrates.Aktactivationistriggeredbybindingofphospholipidandphosphorylationattwokeyresidues:Thr308byPDK1,andSer473byPDK2,nowidentifiedasmTOR.DeregulationofAktsignalinghasbeenassociatedwithcancer,diabetes,andschizophrenia.Akt1isthecellularhomologueofthemurinethymomaretroviraloncogenev-akt,anditsroleinanti-apoptoticandpro-mitoticpathwayshavemadeAktamoleculartargetforanti-cancertherapeuticintervention.AktactivationinhibitsapoptosisbyphosphorylatingtheBcl-2relatedproteinBad,andincreasesp53degradationbyphosphorylatingmdm2.MitoticsubstratesofAktincludeGSK-3β,p21CIP1,andp27KIP1,cellcycleinhibitorsnegativelyregulatedbyAktphosphorylation.Akthasbeenshowntomediateangiogenesisthroughregulationofthrombospondins,whichmaycooperatewithpro-mitoticandanti-apoptoticfunctionsofAkttopromotetumorigenesis. |