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Enzolifesciences/Caspase-7 monoclonal antibody (7-1-11)/ADI-AAM-127-E/100µg

ProductSpecification:

Clone:7-1-11
 
Host:Mouse
 
Isotype:IgG2b
 
Immunogen:RecombinanthumanCaspase-7protein(fulllength).
 
UniProtID:P55210
 
Speciesreactivity:Human,Mouse,Rat
Dog,Hamster,Monkey,Porcine,Rabbit,Sheep
 
Specificity:Detectstheunprocessedpro-caspase-7(p35),caspaselackingtheshortamino-terminalpro-domain(p32)andfullyprocessedcaspase-7(p19)intheapototiccellextract.
 
Applications:IHC(PS),WB
 
RecommendedDilutions/Conditions:WesternBlot(0.2µg/ml,ECL)
Suggesteddilutions/conditionsmaynotbeavailableforallapplications.
Optimalconditionsmustbedeterminedindividuallyforeachapplication.
 
ApplicationNotes:Immunohistochemistry(paraffinsections)
WesternBlot(0.2µg/ml,ECL)
Optimalconditionsmustbedeterminedindividuallyforeachapplication.
 
PurityDetail:ProteinGaffinitypurified.
 
Formulation:Liquid.InPBS,pH7.2,containing0.1mMPMSF.
 
Handling:Avoidfreeze/thawcycles.
 
Shipping:ShippedonBlueIce
 
LongTermStorage:-20°C
 
ScientificBackground:Thecaspasesareafamilyofcysteineproteasesthatcleaveaftercertainaspartateresidues,andareprimarilyrecognizedasmediatorsofapoptosis.caspasesaresynthesizedasinactivezymogensthatcanbecleavedtoformactiveenzymesfollowingtheinductionofapoptosisbystressordeathreceptors.Initiatorcaspases(e.g.caspase-8and-10)areactivatedbydimerizationofthezymogenonadedicatedadaptorprotein.Theseactivatedinitiatorcaspasesin-turncleavedownstreameffectororexecutionercaspases(e.g.caspase-3,-6,and-7)inacascade-likemanner,whichcleavekeycellularproteinsthatleadtothemorphologicalchangesassociatedwithapoptoticcelldeath.
 
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