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| AlternativeName: | Heatshockprotein70,Hsc70,Hsp73 |
| Clone: | 1B5 |
| Host: | Rat |
| Isotype: | IgG2a |
| Immunogen: | NativehamsterHsc70(Hsp73). |
| UniProtID: | P19378 |
| GenBankID: | M34561 |
| Speciesreactivity: | Human,Mouse,Rat Bovine,Chicken,Dog,Guineapig,Hamster,Monkey,Porcine,Rabbit,Sheep |
| Applications: | FlowCytometry |
| RecommendedDilutions/Conditions: | FlowCytometry(10µg/ml) Suggesteddilutions/conditionsmaynotbeavailableforallapplications. Optimalconditionsmustbedeterminedindividuallyforeachapplication. |
| ApplicationNotes: | Detectsabandof~73kDabyWesternblot. |
| PurityDetail: | ProteinGaffinitypurified. |
| Formulation: | Liquid.InPBS,pH7.2,containing0.09%sodiumazide. |
| Handling: | Avoidvigorouscentrifugationandvortexing.Donotfreeze.Protectfromlight. |
| Shipping: | BlueIceNotFrozen |
| LongTermStorage: | +4°C |
| ScientificBackground: | TheHsp70familyofheatshockprotienscontainsmultiplehomologsranginginsizefrom66-78kDa,andaretheeukaryoticequivalentsofthebacterialDnaK.ThemoststudiedHsp70membersincludethecytosolicstress-inducedHsp70(Hsp72),theconstitutivecytosolicHsc70(Hsp73),andtheER-localizedBiP(Grp78).Hsp70familymemberscontainhighlyconservedN-terminalATP-aseandC-terminalproteinbindingdomains.BindingofpeptidetoHsp70isassistedbyHsp40,andstimulatestheinherentATPaseactivityofHsp70,facilitatingATPhydrolysisandenhancedpeptidebinding.Hsp70nucleotideexchangeandsubstratebindingcoordinatesthefoldingofnewlysynthesizedproteins,there-foldingofmisfoldedordenaturedproteins,coordinatestraffickingofproteinsacrosscellularmembranes,inhibitsproteinaggregation,andtargetsthedegradationofproteinsviatheproteasomalpathway. |