Enzolifesciences/Nbr1 (human) polyclonal antibody (fluorescein labeled)/BMLPW11300025/25µl

Description:

Forselectiveautophagyandproteinaggregationstudiesusingfluorescencemicroscopy.

ProductSpecification:

Host:	Rabbit
Immunogen:	Syntheticpeptidecorrespondingtoaa38-51ofhumanNbr1.
UniProtID:	Q14596
Speciesreactivity:	Human
Applications:	ICC
RecommendedDilutions/Conditions:	Immunocytochemistry(1:20) Suggesteddilutions/conditionsmaynotbeavailableforallapplications. Optimalconditionsmustbedeterminedindividuallyforeachapplication.
PurityDetail:	Partiallypurifiedbysaltprecipitation.
Formulation:	Liquid.InPBScontaining0.01%sodiumazide.
Use/Stability:	Stableforatleast1yearafterreceiptwhenstoredat-20°C.Reagentislightsensitive;storeinthedark.Theuseofhighquality‘antiserum-grade’plasticorglassvialsisrecommended.
Handling:	Protectfromlight.Avoidfreeze/thawcycles.Afteropening,preparealiquotsandstoreat-20°C.
Shipping:	ShippedonBlueIce
ShortTermStorage:	+4°C
LongTermStorage:	-20°C
ScientificBackground:	Autophagyisahighlyconservedbulkproteindegradationpathwayresponsiblefortheturnoveroflong-livedproteins,disposalofdamagedorganelles,andclearanceofaggregate-proneproteins.Autophagyisinvolvedinvariousphysiologicalorpathologicalprocesses,suchasdevelopment,hostdefenseresponse,cancerandneuronaldegenerativediseases.Itinvolvesconcertedactionofmorethan20specificautophagy(ATG)proteinsthatmediatetheformationofadouble-membranevesicle,theautophagosome,whichengulfsthesubstrateanddeliversittothelysosomefordegradation. Nbr1(neighborofBRCA1gene1)hasbeenidentified,togetherwithp62,asaselectiveautophagyreceptor,capableofsimultaneouslybindingbothubiquitinandautophagy-specificLC3/GABARAPproteins.Nbr1isrecruitedtoubiquitin-positiveproteinaggregatesanddegradedbyautophagythroughitsinteractionwiththeLC3/GABARAPcomponentoftheautophagosomalmembrane. Suchreceptorproteinsprovideamolecularlinkbetweenubiquitinylationandautophagy,facilitatingtheselectivetargetingofvarioustypesofcargo,rangingfromproteinaggregatestomembrane-boundorganellesandmicrobesforautophagicdegradation.
TechnicalInfo/ProductNotes:	Nbr1polyclonalantibody(Prod.No.BML-PW1125)wasconjugatedtofluoresceinbyaproprietarymethod.Thefluorescein-labelledNbr1polyclonalantibody(Prod.No.BML-PW1130)facilitatesreadydetectionofNbr1inautophagyinhibitedcellsbyfluorescencemicroscopy.