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| AlternativeName: | POU5F1,POUdomainclass5transcriptionfactor1,OTF3/4,Octamer-bindingprotein3/4 |
| Clone: | 9B7 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | RecombinanthumanOct4(aa1-360). |
| UniProtID: | Q01860 |
| Speciesreactivity: | Human |
| Applications: | WB |
| RecommendedDilutions/Conditions: | WesternBlot(1:5,000) Suggesteddilutions/conditionsmaynotbeavailableforallapplications. Optimalconditionsmustbedeterminedindividuallyforeachapplication. |
| ApplicationNotes: | Detectsabandof~39kDa(fulllength)byWesternblot. |
| PurityDetail: | ProteinGaffinitypurified. |
| Formulation: | Liquid.InPBS,pH7.3,containing1%BSA,50%glyceroland0.02%sodiumazide. |
| Shipping: | ShippedonBlueIce |
| LongTermStorage: | -20°C |
| ScientificBackground: | Oct4isamembersofOctfamilyoftranscriptionfactorswhichspecificallyinteractwithOctamermotifATGCAAAT,aregulatoryelementimportantfortissue-andcell-specifictranscriptionaswellasfortranscriptionofanumberofhousekeepinggenes.AllofthemembersoftheOctfamilycontaintwohighlyconserveddomainswhichareseparatedby14-26variableaminoacids.TheseincludethePOUhomeodomainandthePOU-specificdomain.BotharerequiredforDNAbindingandareinvolvedinprotein-proteininteractions.EvidencesindicatethatregulationofOctfamilytranscriptionfactorsoccursatthelevelofphosphorylation.Oct4isrenownedasatranscriptionfactorexpressedbyundifferentiatedembryonicstemcellsandembryonicgermcells. |