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| AlternativeName: | CALR,CRT |
| Host: | Rabbit |
| Immunogen: | Recombinanthumancalreticulin. |
| UniProtID: | P27797 |
| Speciesreactivity: | Human,Mouse,Rat |
| Applications: | WB |
| RecommendedDilutions/Conditions: | WesternBlot(1:1,000,ECL) Suggesteddilutions/conditionsmaynotbeavailableforallapplications. Optimalconditionsmustbedeterminedindividuallyforeachapplication. |
| ApplicationNotes: | Detectsabandof~55kDabyWesternblot. |
| PurityDetail: | ProteinAaffinitypurified. |
| Formulation: | Liquid.InPBScontaining50%glyceroland0.09%sodiumazide. |
| Handling: | Avoidfreeze/thawcycles. |
| Shipping: | ShippedonBlueIce |
| LongTermStorage: | -20°C |
| ScientificBackground: | Themultifunctional,multi-compartmentalproteinCalreticulin(Crt)functionsasasolublemolecularchaperoneofnewormisfoldedproteinsaswellasaCa2+-bindingprotein.MostabundantintheERlumen,Crtexpressionalsooccursinothermembrane-boundorganelles,thecellsurface,andextracellularly.AlsoknownasCRP-55,calregulinandHACBP(highaffinitycalcium-bindingprotein),CrtcontainstheER-retrievalsequence,KDEL,andisthesolubleparalogoftheERmembraneproteinCalnexin(Cnx).Crtsthreedomainsincludea180residueN-terminaldomain,aproline-richP-domain(residues189-288)thatbindsCa2+withhighaffinityandshareshomologywithCnxandcalmegin,anda110residueC-terminaldomainthatbindsCa2+withlowaffinitybuthighcapacity.TheP-domainmayinteractwiththeco-chaperoneERp57(Grp58),athiolreductase.TheNMRstructureoftheP-domainconsistsofanextendedhairpinthatappearstoformacurvedprotrusionfromtheCrtcoredomain.BothCrtanditsmembraneboundhomologCNXinteractwithproteinsandglycoproteinspossessingmonoglucosylatedN-glycans.TheCrt/Cnxcyclepromotescorrectfolding,inhibitsaggregationoffoldingintermediates,blocksprematureoligomerization,regulatesERdegradation,andpreventsincompletelyfoldedglycoproteinsfromexitingtotheGolgicomplex.Crtalsoappearstofunctionasanauto-antigeninsystemiclupuserythematosus,rheumatoidarthritis,celiacdisease,completecongenitalheartblock,andhalothanehepatitis.AdiversityofadditionalfunctionsattributedtoCrtincludesadhesion,bloodfunction,andcardiacandneuronaldevelopmentgeneexpression. |