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| AlternativeName: | Proteasomesubunitβtype-10,MacropainsubunitMECl-1 |
| Host: | Rabbit |
| Immunogen: | Syntheticpeptidecorrespondingtoaa218-236ofmouseproteasome20S(β2isubunit). |
| UniProtID: | O35955(mouse),P40306(human) |
| Gene/ProteinIdentifier: | PSMB10(genename) |
| Speciesreactivity: | Human,Mouse |
| Specificity: | Recognizestheβ2isubunitofthe20Sproteasome. |
| Applications: | IF,IHC,WB |
| ApplicationNotes: | Westernblot:TheserumwascharacterisedbysingledimensionSDS-PAGEagainstmurinederivedproteasomalpreparationsfollowedbyWesternblotting.Aproteinof~29kDawasdetectedusingtheantibodyatadilutionof1:500-1:1000usingenhancedchemiluminescentdetection.Whenamouselivercellderivedlysateisprobedanumberofproteinbandsareobservedinadditiontothatat~29kDa. |
| Formulation: | Liquid.Antiserumcontaining10mMsodiumazide. |
| Use/Stability: | Storeunopenedvialat-20°Cuntilrequiredforuse.AVOIDREPEATEDFREEZE-THAWCYCLES.Aliquotundilutedantibodyintosmallervolumes(notlessthan10μL)priortofreezingifappropriate.Theuseofhighquality‘antiserum-grade’plasticorglassvialsisrecommended.Storedilutedantibodyat2-4°C(donotfreeze)andusewithin1month. DilutetoworkingstrengthwithphosphatebufferedsalinepH7.2-7.4and1%normalgoatserum(ifagoatanti-rabbitIgGlinkerantibodyistobeused). |
| Shipping: | ShippedonBlueIce |
| LongTermStorage: | -20°C |
| ScientificBackground: | Theproteasomeiswidelyrecognisedasthecentralenzymeofnon-lysosomalproteindegradation.Itisresponsibleforintracellularproteinturnoveranditisalsocriticallyinvolvedinmanyregulatoryprocessesand,inhighereukaryotes,inantigenprocessing.The26Sproteasomeisthekeyenzymeoftheubiquitin/ATP-dependentpathwayofproteindegradation.Thecatalyticcoreofthisunusuallylarge(2000kDa,450Åinlength)complexisformedbythe20Sproteasome,abarrelshapedstructureshownbyelectronmicroscopytocompriseoffourringseachcontainingsevensubunits. Basedonsequencesimilarity,allfourteen20Sproteasomalsubunitsequencesmaybeclassifiedintotwogroups,αandβ,eachgrouphavingdistinctstructuralandfunctionalroles.Theα-subunitscomprisetheouterringsandtheβ-subunitstheinnerringsofthe20Sproteasome.Observationsoftheeukaryoticproteasomeandanalysisofsubunitsequencesindicatethateachringcontainssevendifferentsubunits(α7β7β7α7)withamemberofeachsub-familyrepresentedineachparticle.Eachsubunitislocatedinauniquepositionwithintheα-orβ-rings.Lmp2,Lmp7andMECLareinterferongamma-induciblecatalyticsubunitsofthe20Sproteasomewhichmayreplacetheconstitutivecatalyticsubunits,delta,XandZrespectively,duringproteasomebiogenesis.Lmp2andLmp7alterthecleavagesitespecificityofthe20SproteasomeandarerequiredfortheefficientgenerationofTcellepitopesfromanumberofviralproteinsandforoptimalMHCclassIcellsurfaceexpression. |
| TechnicalInfo/ProductNotes: | Speciesreactivity: Thisantibodyhasbeenshowntoreactwithmousederivedmaterialbutitonlyreactsveryweakly,ifatall,withthehumansubunit. Varioussystemsforthenomenclatureoftheproteasomesubunitshavebeenestablished.ThismaybeasourceofconfusionasthesystemonUniProtdiffersfrom"standard"nomenclatureasdescribedintheliterature.TheUniProtIDandGeneNamewillhelptoclearlyidentifytheproteins. |