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| AlternativeName: | MAP2K7,MAPkinasekinase7 |
| Clone: | 10F7 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | SyntheticpeptidecorrespondingtothesequenceneartheaminoterminusofMKK7(MAP2K7)conjugatedtohemocyanin. |
| UniProtID: | O14733 |
| Speciesreactivity: | Human,Mouse,Rat Dog |
| Applications: | ELISA,ICC,IHC,WB |
| RecommendedDilutions/Conditions: | ELISA(0.1µg/ml) Immunohistochemistry(1:50) WesternBlot(0.5µg/ml,ECL) Suggesteddilutions/conditionsmaynotbeavailableforallapplications. Optimalconditionsmustbedeterminedindividuallyforeachapplication. |
| ApplicationNotes: | Detectsabandof~45kDabyWesternblot. |
| PurityDetail: | Thiophilicadsorptionandsizeexclusionchromatographypurified. |
| Formulation: | Lyophilizedfrom1mlof2xPBScontaining0.09%sodiumazide,PEG,andsucrose. |
| Reconstitution: | Reconstitutewith1mlwater(15minutesatroomtemperature). |
| Use/Stability: | Stableat-80°Cupto1year,at4°Cupto3months. |
| Shipping: | ShippedonBlueIce |
| LongTermStorage: | -20°C |
| ScientificBackground: | MembersoftheMAPkinasefamilyofproteinkinases(e.g.p38andJnk)aresubjecttodualphosphorylationofaconservedT-X-YmotifintheiractivationdomainbyupstreammembersoftheMAP/ERKkinase(MEK/MKK)family.MKK3andMKK6phosphorylatep38inresponsetocytokinestimulationandcellularstress,whileMKK7andMKK4activatetheJnkkinasesbyphosphorylationofthethreonineandtyrosineresidues,respectively. |