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| AlternativeName: | Checkpointkinase1 |
| Clone: | DCS-310 |
| Host: | Mouse |
| Isotype: | IgG3 |
| Immunogen: | RecombinanthumanChk1. |
| UniProtID: | O14757 |
| Speciesreactivity: | Human,Mouse,Rat |
| Applications: | IHC,IP,WB |
| RecommendedDilutions/Conditions: | Immunohistochemistry(1µg/ml) Immunoprecipitation(2µg/200µlcellextract,5x106cells) WesternBlot(1µg/ml,ECL) Suggesteddilutions/conditionsmaynotbeavailableforallapplications. Optimalconditionsmustbedeterminedindividuallyforeachapplication. |
| ApplicationNotes: | Detectsabandof~55kDabyWesternblot. |
| PurityDetail: | ProteinAaffinitypurified. |
| Formulation: | Liquid.InPBS,pH7.2,containing50%glycerol. |
| Shipping: | ShippedonBlueIce |
| LongTermStorage: | -20°C |
| ScientificBackground: | TheDNAdamagecheckpointisasignaltransductionpathwaythathastheabilitytodelayentryintomitosis.WhenDNAisdamaged,theCheckpointkinases(CHKs)areknowntoactdownstreamofATMtoelicitappropriateresponsessuchascellcyclearrest.Chk1,forinstance,isactivatedbyATMphosphorylationofserines123,178,278,and292oftheSphase-promotingcdc25AphosphataseandacceleratesIR(ionizingradiation)induceddegradationofcdc25A. |