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| AlternativeName: | Multidrugresistance-associatedprotein2,cMOAT,ABCC2 |
| Clone: | M2III-6 |
| Host: | Mouse |
| Isotype: | IgG2a |
| Immunogen: | Recombinant C-terminalrat MRP2(multidrugresistance-associatedprotein-2;cMOAT)(aa1339-1541). |
| UniProtID: | Q63120 |
| Speciesreactivity: | Human,Rat |
| Specificity: | RecognizesaninternalepitopeofMRP2. |
| Crossreactivity: | Doesnotcross-reactwithhumanP-glycoprotein,MDR3,MRP1,MRP3orMRP5. |
| Applications: | ICC,IHC(FS),IHC(PS),WB |
| RecommendedDilutions/Conditions: | Immunocytochemistry:Use1:20-1:50dilutiononacetonefixedcytospinpreparations.,Immunohistochemistry(frozensections)(1:20):Canbeuseonacetonefixedfrozensections.Canbefollowedbyincubationwithrabbitanti-mouseantibodyandamonoclonalmouseAPAAPcomplex. Immunohistochemistry(paraffinsections)(1:20,weak):Canbeusedonformaldehyde-fixedparaffin-embeddedtissuesandtumors,afterpretreatmentwith0.01Mcitricacid(pH6.0)indistilledwaterat100°Cfor5min3times.Afterincubationwiththeprimaryantibodyandwashing,slidescanbeincubatedwithbiotinylatedrabbitanti-mouseantibodyandstreptavidinconjugatedtohorseradishperoxidase. WesternBlot:Use1:20-1:50andanti-mouseHRP. Suggesteddilutions/conditionsmaynotbeavailableforallapplications.Optimalconditionsmustbedeterminedindividuallyforeachapplication. |
| Formulation: | Liquid.Inserumfreeculturesupernatantcontaining0.7%BSAand0.1%sodiumazide. |
| Handling: | Avoidfreeze/thawcycles. |
| Shipping: | ShippedonBlueIce |
| ShortTermStorage: | +4°C |
| LongTermStorage: | -20°C |