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| AlternativeName: | AtCUL1 |
| Host: | Rabbit |
| Immunogen: | FulllengthrecombinantAtCUL1. |
| UniProtID: | Q94AH6 |
| Speciesreactivity: | Arabidopsisthaliana |
| Applications: | WB |
| RecommendedDilutions/Conditions: | WesternBlot(1:1,000) Suggesteddilutions/conditionsmaynotbeavailableforallapplications. Optimalconditionsmustbedeterminedindividuallyforeachapplication. |
| Formulation: | Liquid.Neatserumcontaining0.1%sodiumazide. |
| Use/Stability: | Storeunopenedvialat-20°Cuntilrequiredforuse.Storedilutedantibodyat4°C(donotfreeze)andusewithin1month. |
| Handling: | Avoidfreeze/thawcycles. |
| Shipping: | ShippedonBlueIce |
| ShortTermStorage: | -20°C |
| LongTermStorage: | -20°C |
| ScientificBackground: | AseriesofstudiesinCaenorhabditiselegans,Schizosaccharomycespombeandmammalshasshedlightontheimportantrolesthatcullin-basedubiquitinligasesplayintheregulationofdiversedevelopmentalprocessesandenvironmentalresponsesineukaryoticorganisms.Theubiquitin-likeproteinRUB1(NEDD8inmammals)isconjugatedtotargetproteinsbyamechanismsimilartothatofubiquitinconjugationinArabidopsis.IthasbeenshownthattheSkp1-Cul1/Cdc53-F-box(SCF)subunitCUL1ismodifiedbyRUB1invivo.TheformationofRUB-AtCUL1isdependentonAXR1andECR1andexpressionoftheseproteinsisrestrictedtozonesofactivecelldivisionandcellelongation,consistentwiththeirroleingrowthregulation.TheseresultsprovidestrongsupportforamodelinwhichRUBconjugationofAtCUL1affectsthefunctionofSCFE3s. |