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| Clone: | P4D1 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Denaturedbovineubiquitin. |
| UniProtID: | P0CG53(UBB),P0CH28(UBC),P62992(RPS27A),P63048(UBA52) |
| Source: | Purifiedfromtissueculturesupernatant.HRPconjugatedchemicallyinvitro. |
| Speciesreactivity: | Speciesindependent |
| Specificity: | Recognizesmono-andpoly-ubiquitinproteinconjugates,freepolyubiquitinchainsandfreeubiquitin. |
| Applications: | WB |
| RecommendedDilutions/Conditions: | WesternBlot(1:1,000) Suggesteddilutions/conditionsmaynotbeavailableforallapplications. Optimalconditionsmustbedeterminedindividuallyforeachapplication. |
| PurityDetail: | Ionexchangepurified. |
| Formulation: | Liquid.InPBScontaining0.01%sodiumazide. |
| Handling: | Avoidfreeze/thawcycles. |
| Shipping: | BlueIceNotFrozen |
| ShortTermStorage: | +4°C |
| LongTermStorage: | +4°C |
| ScientificBackground: | Ubiquitinmodificationofcellularproteinshasbeenshowntobeinvolvedwithawiderangeofbiochemicalprocessesincludingproteasomaldegradation,signaltransduction,DNArepair,endocytosisandautophagy. |
| TechnicalInfo/ProductNotes: | P4D1antibodywasdevelopedusingadenaturedglutaraldehydecross-linkedubiquitinIgGcomplexastheimmunogen.Monoclonalantibodywaspurifiedfromtissueculturesupernatantbyionexchangechromatography.ThegeneralubiquitinreactivityofthemonoclonalantibodyhasbeendemonstratedbyWesternblottingusingsyntheticubiquitinchains,singleisopeptide-linkedpolyubiquitinchains,andwithendogenouslyubiquitinylatedspeciesincelllysates. |