企业QQ
官方微信
| AlternativeName: | Hsp70,HspA1A,Heatshockprotein70,HspA1B,Hsp72 |
| Clone: | N31F2-4 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | NativehumanHsp70. |
| UniProtID: | P0DMV8(HSPA1A),P0DMV9(HSPA1B) |
| GenBankID: | M11717 |
| Gene/ProteinIdentifier: | NP_005336.3(RefSeq),NM_005345(RefSeq),3303(EntrezGeneID),140550(OMIM) |
| Speciesreactivity: | Human E.coli |
| Applications: | WB |
| RecommendedDilutions/Conditions: | WesternBlot(1:1,000,ECL) Suggesteddilutions/conditionsmaynotbeavailableforallapplications. Optimalconditionsmustbedeterminedindividuallyforeachapplication. |
| ApplicationNotes: | Detectsabandof~70kDabyWesternblot. |
| PurityDetail: | ProteinGaffinitypurified. |
| Formulation: | Liquid.InPBScontaining50%glyceroland0.09%sodiumazide. |
| Handling: | Avoidfreeze/thawcycles. |
| Shipping: | ShippedonBlueIce |
| LongTermStorage: | -20°C |
| ScientificBackground: | The70kDaheatshockproteinHsp70belongstotheHsp70familyofhighly-relatedproteinisoformsranginginsizefrom66kDato78kDa.Hsc70sharesclosebiochemicalandbiologicaltiestoHsp70,andalsobelongstotheHsp70family.Theseproteinsincludecognatemembersfoundwithinmajorintracellularcompartmentsandhighlyinducibleisoformspredominantlycytoplasmicornuclearindistribution.MembersoftheHsp70familyfunctionasmolecularchaperonesinvolvedinsuchcellularfunctionsasproteinfolding,transport,maturationanddegradation,operatinginanATP-dependentmanner.ThemolecularchaperonesoftheHsp70familyrecognizeandbindtonascentpolypeptidechainsorpartiallyfoldedintermediatesofproteins,preventingtheiraggregationandmisfolding,andthebindingofATPtriggersacriticalconformationalchangeleadingtothereleaseoftheboundsubstrateprotein.Datademonstratesthatwithaubiquitin-likedomainatitsaminoterminusanditsassociationwiththe26SproteosomeinHeLacells,Bag-1modulatesthechaperoneactivityofHsc70andHsp70.ThesefindingsrevealBag-1sroleasaphysicallinkbetweentheHsc70/Hsp70chaperonesystemandtheproteasome.ExperimentaldataalsoshowsthattheATPasedomainandthesubstrate-bindingdomainofHsp70(orHsc70)cooperatetoformaco-chaperone-chaperonecomplexwiththesynapticvesiclecysteinestringprotein(csp),essentialfornormalneurotransmitterrelease. |