Enzolifesciences/Urm1 (human) polyclonal antibody/BMLPW95950025/25µl

首页 Enzo Life Sciences Proteasome activator 11S γ subunit polyclonal antibody Welk V, Coux O, Kleene V, Abeza C, Trumbach D, Eickelberg O, et al. Inhibition of Proteasome Activity Induces Formation of Alternative Proteasome Complexes. J Biol Chem. 2016;291:13147-59 pubmed 出版商 背景: The proteasome is widely recognised as the central enzyme of non-lysosomal protein degradation. It is responsible for intracellular protein turnover and it is also critically involved in many regulatory processes and, in higher eukaryotes, in antigen processing. The 26S proteasome is the key enzyme of the ubiquitin/ATP-dependent pathway of protein degradation. The catalytic core of this unusually large (2000kDa, 450?? in length) complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. 20S Proteasomes degrade only unfolded proteins in an energy-independent manner, whereas 26S proteasomes degrade native and ubiquitinylated proteins in an ATP-dependent manner. The native protein substrates are recognised by subunits, some with ATP binding sites, of the outer 19S caps of the 26S proteasome.A second activator which can associate with the 20S proteasome in the absence of ATP is known as PA28 or the 11S regulator. The pure PA28 activator is a complex of two alternating subunits, PA28α and PA28β, which share approximately 50% homology but also show considerable similarity (30-40%) to a nuclear protein of unknown function, the Ki autoantigen (now referred to as PA28γ). These subunits, with an apparent relative molecular weight of approximately 29kDa, form ringlike heteromeric complexes of ~200kDa possibly with an α3β3 stoichiometry. Electron microscopic studies have shown PA28 to be a ring shaped particle which, like the 19S, caps the 20S proteasome, by binding to the α-rings, at both or either end. The complex may, however, be readily dissociated. The finding that PA28 modulates the proteasome-catalysed production of antigenic peptides presented to the immune system on MHC class I molecules indicates a cellular function of this activator in antigen processing. 别名: Proteasome activator complex subunit 3, PA28γ 免疫原: Synthetic peptide corresponding to aa 70-85 of human and mouse Ki autoantigen/PA28γ. 特异性: Recognizes the γ subunit of proteasome activator 11S. 缓冲液: Liquid. Antiserum containing 10mM sodium azide. 用途: Detects a band of ~31kDa by Western blot. 应用摘要: 免疫组化, 免疫印迹 储存: -20°C 更多信息或购买: Enzo Life Sciences 产品网页 techserv-usa@enzolifesciences.com www.enzolifesciences.com1-800-942-0430 公司总部: 美国 自2007年始，Enzo Life Sciences先后合并ALEXIS Biochemicals、BIOMOL International等公司，为生命科学研究各个领域提供更全面的试剂产品。比如，肿瘤、细胞死亡、表观遗传、基因组学、免疫学、神经科学、信号转导及泛素和蛋白酶体等领域研究，都可以在Enzo Life Sciences中找到相应抗体、酶、蛋白、小分子抑制剂、产品文库及检测kit等产品。