企业QQ
官方微信
| Formulation | 50%glycerol/water(v/v) |
| Storage | -20°C |
| Purity | >95%bySDS-PAGE |
| ActivityDetermination | Chromogenicassay |
| ShelfLife(properlystored) | 12months |
| Gel | Novex4-12%Bis-Tris |
|---|---|
| Load | HumanPlasmin,1µgperlane |
| Buffer | MES |
| Standard | SeeBluePlus2;Myosin(191kDa),PhosphorylaseB(97kDa),BSA(64kDa),GlutamicDehydrogenase(51kDa),AlcoholDehydrogenase(39kDa),CarbonicAnhydrase(28kDa),MyoglobinRed(19kDa),Lysozyme(14kDa) |
Plasminogen(whetherGlu-1,Lys-77orVal-442)isconvertedtotheactiveserineproteaseplasminbyhydrolysisoftheArg560-Val561peptidebondyieldinganNH2-terminalheavy(A)chainandaCOOH-terminallight(B)chainlinkedby2disulfidebonds(1-3).Thisconversioniscatalyzedbyavarietyofphysiologicalandpathologicalactivators,includingurinarytypeplasminogenactivators,tissuetypeplasminogenactivators,streptokinase,staphylokinase,kallikrein,factorsIXaandXIIa.TheCOOH-terminalderivedlightchain(Mr=26,000)containsthecatalytictriad(His42,Asp85andSer180)aswellasthestreptokinasebindingsite.TheNH2-terminalderivedheavychainrangesinmolecularweightfrom63,000to12,000dependingonthetypeofplasminogenfromwhichitoriginated.Intheabsenceofinhibitors,plasmincleavestheamino-terminalGlu1toLys76peptidefromplasmin(plasminogen)toyieldLys-plasmin,whichhasagreateraffinityforfibrinthantheGluform.TheheavychainofLys-plasminogencontains5tripleloopdisulfidebridgedregionsofinternalsequencehomologyknownaskringles.Kringles1-4containtheω-aminocarboxylicacidandfibrinbindingsites.
Plasminisaserineproteasewithbroadspecificitywhich,inadditiontocleavageoffibrin,iscapableofactivationand/ordegradationofcompoundsofthecoagulation,kiningenerationandcomplementsystems.Althoughplasmincanbeinhibitedbyanumberofplasmaproteaseinhibitorsinvitro,regulationofplasmininvivoisthoughttooccurmainlythroughitsinteractionwithα2-antiplasmin,andtoalesserextent,α2-macroglobulin.
HumanLys-plasminispreparedfromhomogeneousGlu-plasminogenusingurokinase,asdescribedbyRobbinsetal.(3).Plasminissuppliedin50%(vol/vol)glycerol/H2Oandshouldbestoredat-20°C.ActivityismeasuredbychromogenicsubstrateassayandpurityisjudgedbySDSgelelectrophoresis.
| Localization | Plasma | |||||
|---|---|---|---|---|---|---|
| Modeofaction | Enzymeinvolvedinfibrinolysis | |||||
| Molecularweight | 83,000(lys-plasmin)(4) | |||||
| Extinctioncoefficient |
| |||||
| Isoelectricpoint | 6.7-8.3(3) | |||||
| Structure | Twosubunits,Mr=57,000andMr=26,000,disulfidelinked,5kringledomains22disulfidebridges,NH2-terminallysine. | |||||
| Percentcarbohydrate | Approximately2%(2) |
matriks/Shikari® (S-ATP) Anti-Pembrolizumab ELISA w/confirmation/PEM-QNS-KEY
intactgenomics/Methionine Auxotrophic LBA4404 | ig® | Intact Genomics/15x50μl/1076-15
goprolytix/Proteolytic Activation of Prothrombin/1 mg, 200 µg/BCT-DFP
novateinbio/Dynorphin A ( 13-17 ) Porcine Peptide/5mg/PE-54024_5mg
matriks/Shikari® (Q-ETA) Etanercept ELISA/ETA-FD-ENB
intactgenomics/Agrobacterium ElectroComponent Combo Pack | Intact Genomics/12x50µl/1290-24
Jackson/Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)/2.0 ml/115-035-003
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
Qubit™ dsDNA BR Assay Kit Q32853现货
Invitrogen Q32855 Qubit™ RNA HS Assay Kit现货促销
LUCK-1G,D-Luciferin, Potassium Salt 钾盐现货
GoldBio/D-Luciferin, Sodium Salt (Proven and Published™)/LUCNA-1G/1 g