dTAG13 Supplier | CAS 2064175411 | dTAG13 | Tocris Bioscience

说明：DegradesmutantFKBP12F36Vfusionproteins;usefulalternativetogeneticmethodsfortargetvalidation化学名：1-[(2S)-1-Oxo-2-(3,4,5-trimethoxyphenyl)butyl]-(2S)-2-piperidinecarboxylate(1R)-3-(3,4-dimethoxyphenyl)-1-[2-[2-[[6-[[2-(2,6-dioxo-3-piperidinyl)-2,3-dihydro-1,3-dioxo-1H-isoindol-4-yl]oxy]hexyl]amino]-2-oxoethoxy]phenyl]propylester纯度：98%(HPLC)
生物活性

PROTACtargetingmutantFKBP12F36Vfusionproteins.ComprisesaligandselectiveforF36Vsingle-pointmutatedFKBP12,alinkerandacereblon-bindingligand.FKBP12F36Vcanbeexpressedasafusionwithatargetproteinofinterestusinggenomeengineeringtechniques(viatransgeneexpressionorCRISPR-mediatedlocus-specificknock-in).ApplicationofdTAG-13inducesrapid,reversibleandselectivedegradationofFKBP12F36Vfusionproteinsinvitroandinvivo.dTAGisgeneralizabletoarangeoffusionproteins;usefulasanalternativetogeneticmethodsfortargetvalidation.

PlasmidvectorsforthelentiviralexpressionandCRISPR-mediatedknock-inofFKBP12F36VareavailablefromAddgene.

CC[C@@H](C1=CC(OC)=C(C(OC)=C1)OC)C(N2CCCC[C@H]2C(O[C@@H](C3=C(C=CC=C3)OCC(NCCCCCCOC4=C5C(N(C(C5=CC=C4)=O)C6CCC(NC6=O)=O)=O)=O)CCC7=CC(OC)=C(C=C7)OC)=O)=O

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

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以下数据基于产品分子量1049.18。Batchspecificmolecularweightsmayvaryfrombatchtobatchduetothedegreeofhydration,whichwillaffectthesolventvolumesrequiredtopreparestocksolutions.
MolarityCalculatorCalculatethemass,volume,orconcentrationrequiredforasolution.

*Whenpreparingstocksolutionsalwaysusethebatch-specificmolecularweightoftheproductfoundontheviallabelandSDS/CoA(availableonline).

Thereconstitutioncalculatorallowsyoutoquicklycalculatethevolumeofareagenttoreconstituteyourvial.Simplyenterthemassofreagentandthetargetconcentrationandthecalculatorwilldeterminetherest.

关键词:dTAG-13,dTAG-13supplier,dTAG13,degraders,degrades,degradation,FKBP12F36V,fusion,protein,mutant,PROTAC,proteolysis,targeting,chimera,TAG,Degradation,Platform,6605,TocrisBioscience

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$50/€35/£30/$50CAN/¥300Yuan/¥5000Yenforfirsttoreviewwithanimage

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$10/€7/£6/$10CAD/¥70Yuan/¥1110Yenforareviewwithoutanimage

AssayeddifferentdTagconcentrations(50nm,250nm,500nm)fortheknockdownofaFKBP-taggedchromatin-boundprotein(180kDa).Wholecelllysate(RIPA)revealednochangetoWTcells(150kDa)andconsiderableknockdowninallconditionsafterjustanhouroftreatment.

UseddTAG-13todegradeatransgenetaggedwithFKBM12-F36VandHAtagstovisualizetheprotein.

ThisisawesternblotprobedforHAtagtotrackdegradation.Allsamplesaretreatedwith500nMdTAG-13.Lanesare:1)parentalcellline,2)control(notreatment),3)30minsoftreatment,4)1hroftreatment,5)2hrsoftreatment,6)4hrsoftreatment,7)6hrsoftreatment.Theconstructs,asshownbythewestern,wasdegradedalmostentirely(98%quantified),within30mins.Ihavetestedbatch2ofthisproductatconc.aslowas50nMandstilljustaseffective.Noapparenttoxicitytocells.Extremelysatisfied.

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FirstgenerationDegraderformutantFKBP12F36Vfusionproteins;usefulalternativetogeneticmethodsfortargetvalidation
Cat.No.6971DegraderofMTH1fusionproteinsforusewithintheaTAGsystem

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