CHIR 99021 ≥98% (HPLC) | 4423 | Tocris Bioscience

化学名：6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethyl]amino]-3-pyridinecarbonitrile纯度：98%(HPLC)
生物活性

Potentandhighlyselectiveinhibitorofglycogensynthasekinase3(GSK-3)(IC50valuesare6.7and10nMforGSK-3andGSK-3respectively).Exhibits500-foldselectivityforGSK-3overcloselyrelatedkinases;alsodisplays800-foldselectivityagainst45additionalenzymesandreceptors.Incombinationwithtranylcypromine(Cat.No.3852),enablesreprogrammingofmouseembryonicfibroblasts,transducedbyOct4andKlf4only,intoiPSCs.EnhancesmouseandhumanESCself-renewalwhenusedincombinationwithPD0325901(Cat.No.4192).Allowsformationofextendedpluripotentstem(EPS)cellsincombinationwith(S)-(+)-Dimethindenemaleate(Cat.No.1425),minocyclinehydrochloride(Cat.No.3268)andhumanleukemiainhibitoryfactor.

CHIR99021synthesizedtocGMPguidelinesalsoavailable.

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocrisproductsareintendedforlaboratoryresearchuseonly,unlessstatedotherwise.

以下数据基于产品分子量465.34。Batchspecificmolecularweightsmayvaryfrombatchtobatchduetothedegreeofhydration,whichwillaffectthesolventvolumesrequiredtopreparestocksolutions.
MolarityCalculatorCalculatethemass,volume,orconcentrationrequiredforasolution.

*Whenpreparingstocksolutionsalwaysusethebatch-specificmolecularweightoftheproductfoundontheviallabelandSDS/CoA(availableonline).

Thereconstitutioncalculatorallowsyoutoquicklycalculatethevolumeofareagenttoreconstituteyourvial.Simplyenterthemassofreagentandthetargetconcentrationandthecalculatorwilldeterminetherest.

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IfyouknowofarelevantreferenceforCHIR99021,pleaseletusknow.

关键词:CHIR99021,CHIR99021supplier,CHIR99021,glycogen,synthase,kinase,3,gsk-3,gsk-3a,gsk-3alpha,gsk-3,gsk-3b,gsk-3beta,gsk-3,inhibitors,inhibits,highly,selective,potent,organoids,Wnt,agonist,Glycogen,Synthase,Kinase,Stem,Cell,Reprogramming,Organoids,4423,TocrisBioscience

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Usedthisproductroutinelytodifferentiatedopaminergicneuronsfromhumanstemcells.

AlliPSClinesandR1mouseEScellsweremaintainedonfeedersinKO-DMEM(Invitrogen)with3mMCHIR99021,and1mMPD0325901..Anonymouson12/04/2019

AlliPSClinesandR1mouseEScellsweremaintainedonfeedersinKO-DMEM(Invitrogen)with3mMCHIR99021,and1mMPD0325901.

CulturemouseEScellsin2imediaandwithout2itoassessdifferentiation.Onlythecellsin2iarepluripotentwhilemEScellsculturedin-2ifor48hoursexitnaivepluripotency.

Fordirecteddifferentiationofpluripotentstemcellstokidneyorganoids,cellsaretreatedwithGSK-3inhibitor,CHIR99021toinducecanonicalWNTsignaling.CHIR99021concentrationanddifferentiationtimeperiodarethekeyfactorsforthisprotocol.Ifoundthisproductgavemereproducibleresults.

Althoughthedatasheetsaysstoreat-20°C,it"sbettertoaliquotandkeepitat-80°Cforlongtermstorage.

CHIR99021实验方案

ThefollowingprotocolsfeatureadditionalinformationfortheuseofCHIR99021(Cat.No.4423).

Tocris提供以下领域的科研文献，以展现我们的产品。您不妨现在就索取*或下载所需的文献！

*请注意，Tocris仅会向正规科研企业/机构地址发送文献。

OurGMPstemcellcompoundsaresuitableforuseasancillaryreagents(rawmaterials)forthefurthermanufacturingofstemcelltherapies.

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