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IBL/Epstein-Barr virus EA IgM ELISA/RE56231/

Kitsize12x8
MethodELISA
Incubationtime1x1h,1x30min,1x20min
Standardrange0-200U/mL,cut-off10U/mL
Specimen/Volumes5µLserum,plasma
Substrate/isotopeTMB450nm
RegulatoryStatus:EU:CE
Detailsfor: Epstein-BarrvirusEAIgMELISA
InfectiousmononucleosisisanacutelymphoproliferativediseasethatiscommoninchildrenandyoungadultsandiscausedbytheEpstein-Barrvirus.TheEBVisoneoftheherpesviruses4(gamma).
Characteristicclinicalfeaturesinclude:
1.fever,sorethroat,andlymhadenopathy,
2.anassociatedabsolutelymphocytosisgreaterthan50%containingatleast10%ofatypicallymphocytesintheperipheralblood,
3.developmentoftransientheterophilandpersistentantibodyresponsesagainstEBV,
4.andabnormalliverfunctiontests.
4%ofinfectedyoungadultsshowanictericmanifestationand50%havesplenomegaly.Inaddition,EBVisimplicatedinBurkittlymphoma,nasopharyngealcarcinomaandHodgkin´sdisease.
Asyndromesimilartoinfectiousmononucleosiscanbecausedbycytomegalovirus,toxoplasmosisandotherviralinfections.Thereforethedifferentialdiagnosisisofmajorimportance.SEROlogicaltestslikeEIAareveryusefulforthedetectionofanti-EBVIgGandIgMantibodies,especiallyincaseswhereheterophilantibodiesareabsent.InafreshinfectionIgMantibodiesagainstVCAandEAaredeterminedbyimmunofluorescenceorELISA.LateronVCAIgGappearfollowedbyEBNA-1IgGantibodies.CorrespondinglythesimultaneousactivationofVCAIgMandEBNA-1IgGindicatesareactivationofanEBVinfection.TheIBLEBV(EA)IgGELISAishelpfultomonitorconvalescenceandreactivatedinfectionsaswellasthedetectionofthenasopharynxcarcinomaandBurkittLymphoma.ImmuneresponsestothenasopharynxcarcinomaandchronicreactivatedEBVinfectionscanbecharacterizedwiththehelpoftheIBLEBV(EA)IgAELISA.
ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.
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