产品资料

Novus/Human CCL20/MIP-3 alpha ELISA Kit (Colorimetric) (DDXK-E-MIP-3-alpha)/DDXK-E-MIP-3-alpha/1 Kit

Summary:

Specificity	ThesecloneswereusedtosetupahumanMIP3-alphaspecificELISA,whichalsodetectsmacaqueMIP3alpha.Nocross-reactivitywithMIP1alpha,MIP1Beta,MIP1delta,MIP3beta,eotaxin,6Ckine,Rantes,MCP-1,TARC,MDC,TECK,SDF-1andIP-10wasdetected(Dieu-Nosjeanetal,J;Exp.med,2000192:705-18).
StandardCurveRange	0.01-20ng/ml
Sensitivity	TheminimumdetectabledoseofhumanMIP3awasdeterminedtobeapproximately0.2ng/ml.
AssayType	SandwichELISA
SampleVolume	100uL
KitType	ELISAKit(Colorimetric)
Gene	CCL20

Applications/Dilutions:

ApplicationNotes

ThehumanMIP-3alphaELISAkitisforquantitativedeterminationofMIP3alphaconcentrationsinhumancellculturesupernatantsandBIOLOGicalfluids(plasma,serum).Thiskitisforresearchpurposeonly.

Publications

ReadPublicationsusingDDXK-E-MIP-3-alpha.

Packaging,Storage&Formulations:

Components

DetectionAntibody:0.5mg/mLofHRPO-conjugatedanti-MIP3alphamousemonoclonalantibody(clone206D9).
Standard:Eachvialcontains1g/mLofrecombinanthumanMIP3alphaproduced,purifiedandconcentratedfromeukaryoticcells.A7-pointstandardcurveusing2-foldserialdilutionsinsampledilutionbuffer,andahighstandardof20ng/mLisrecommende
CaptureAntibody:0.5mg/mLofmouseanti-MIP3alphamonoclonalantibody(clone319F6).

Notes:

ThisproductismanufacturedbyDendriticsanddistributedbyNovusBiologicalsinNorthAmericaandotherselectcountries.Toorderinthefollowingcountries:Germany,Finland,UnitedKingdom,Norway,Denmark,Switzerland,AustriaandSouthKorea,pleasecontactDendriticsforadditionalorderinginformation.

AlternateNamesfor53BP1Antibody:

betachemokineexodus-1
Beta-chemokineexodus-1
CCchemokineLARC
C-Cmotifchemokine20
CCL20
chemokine(C-Cmotif)ligand20
CKb4
exodus-1
LARC
LARCLiverandactivation-regulatedchemokine
MIP3alpha
MIP-3alpha
MIP-3a
MIP-3-alpha
MIP3AMacrophageinflammatoryprotein3alpha
SCYA20Small-inducIBLecytokineA20
smallinduciblecytokinesubfamilyA(Cys-Cys),member20
ST38

Background:

Macrophageinflammatoryprotein(MIP-3a)/CCL20isaCC-typechemokinemappedtochromosome2inhumans.Langerhanscells(LCs)representauniquepopulationofDCscolonizingepithelium.MIP-3aplaysacentralroleinLCprecursorrecruitmentintotheepitheliumduringinflammation.(a)AmongDCpopulations,MIP-3aisthemostpotentchemokineinducingtheselectivemigrationofinvitro-generatedCD34+hematopoieticProgenitorcell-derivedLCprecursorsandskinLCs,inaccordancewiththerestrictedMIP-3areceptor(CCchemokinereceptor6)expressionbythesecells.(b)MIP-3aismainlyproducedbyepithelialcells,andthemigrationofLCprecursorsinducedbythesupernatantofactivatedskinkeratinocytesisblockedbyanantibodyagainstMIP-3a.(c)Invivo,MIP-3aisselectivelyproducedatsitesofinflammationasillustratedintonsilsandlesionalpsoriaticskinwhereMIP-3aupregulationisassociatedwithanincreaseinLCturnover.(d)ThesecretionofMIP-3aisstronglyupregulatedbycellsofepithelialoriginafterinflammatorystimuli(interleukin1bplusTNFa)orTcellsignals(HomeyB.etal,2000,J.Immunol.164:6621-32).Monoclonalantibodiesanti-MIP3awereobtainedaftermiceimmunizationwithMIP-3a-transfectedeukaryoticcells.ThesecloneswereusedtosetupahumanMIP-3aspecificElisa,whichalsodetectmacaqueMIP3a.Nocross-reactivitywithMIP-1a,MIP-1b,MIP-1d,MIP-3b,eotaxin,6Ckine,Rantes,MCP-1,TARC,MDC,TECK,SDF-1andIP-10wasdetected(Dieu-Nosjeanetal,J;Exp.med,2000192:705-18).

Limitations:

betachemokineexodus-1
Beta-chemokineexodus-1
CCchemokineLARC
C-Cmotifchemokine20
CCL20
chemokine(C-Cmotif)ligand20
CKb4
exodus-1
LARC
LARCLiverandactivation-regulatedchemokine
MIP3alpha
MIP-3alpha
MIP-3a
MIP-3-alpha
MIP3AMacrophageinflammatoryprotein3alpha
SCYA20Small-induciblecytokineA20
smallinduciblecytokinesubfamilyA(Cys-Cys),member20
ST38

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