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Novus/PiColorLock™ Phosphate Detection Reagent/303-0030

Description
PiColorLock phosphate detection reagent changes colour in the presence of inorganic phosphate (Pi). This can be exploited to measure any enzyme that generates Pi including ATPases, GTPases, phosphatases, heat-shock proteins and DNA unwinding proteins.The unique formulation of PiColorLock affords enhanced assay linearity, dynamic range and colour stability; it is also possible with PiColorLock to work with unstable substrates (e.g. ATP, GTP) that give high non-enzymatic background signals with other acidic dye-based detection reagents.
FeaturesBenefits
Colorimetric assaySimple, scalable and non-radioactive
Compatible with almost any assay bufferFlexibility over a wide range of existing assays
Stable reagent formulationLong shelf life
Unique accelerator and stabilizerSpeeds up color development
PiColorLock-Pi complex is very stableNo precipitation, results can be measured over several hours - ideal for HTS
Wide linear rangeNo inhibition of color development by high concentrations of protein
Reviewed Applications
Read 3 Reviews rated 5
using 303-0030 in the following application:
  • Immunocytochemistry/Immunofluorescence
  • Serum
  • ELISA
Publications
ReadPublications using 303-0030 in the following applications:
  • EnzAct
    1 publication
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