产品资料

Novus/Recombinant Human FGF-4 Protein (NBP2-34864)/NBP2-34864-5ug/5 ug

Summary:

Description	ABIOLOGicallyactiveproteintoFGF4. Source:E.coli AminoAcidSequence:GRGGAAAPTAPNGTLEAELERRWESLVALSLARLPVAAQPKEAAVQSGAGDYLLGIKRLRRLYCNVGIGFHLQALPDGRIGGAHADTRDSLLELSPVERGVVSIFGVASRFFVAMSSKGKLYGSPFFTDECTFKEILLPNNYNAYESYKYPGMFIALSKNGKTKKGNRVSPTMKVTHFLPRL
Preparation Method	Novus"biologicallyactiveproteinsarestringentlypurifiedtoprovideonlythesafestandmosthighlyeffectiveproteinsavailable.ThisproteinwasexpressedinE.coli,purifiedbyHPLC,QCtestedbySDS-PAGEandWesternBlotandvalidatedonappropriatecelllinesforbioactivity.AllHPLCandbioactivitydataisprovidedforyourassurance.
DetailsofFunctionality	FibroblastGrowthFactor4proteinisfullybiologicallyactivewhencomparedtostandard.TheED50asdeterminedbythymidineuptakeassayusingFGFreceptorstransfectedBaF3cellsislessthan0.5ng/ml,correspondingtoaspecificactivityof>2.0x106IU/mg
Protein/PeptideType	BiologicallyActiveProtein
Gene	FGF4
Purity	>95%purebySDS-PAGE
EndotoxinNote	Lessthan1EU/ugofendotoxinasdeterminedbyLALmethod.

Applications/Dilutions:

TheoreticalMW

19.8kDa.
Disclaimernote:Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors.

ReviewedApplications

Read1Reviewrated5

using
NBP2-34864inthefollowingapplication:

Packaging,Storage&Formulations:

Thislyophilizedpreparationisstableat2-8degreesC,butshouldbekeptat-20degreesCforlongtermstorage,preferablydesiccated.Uponreconstitution,thepreparationismoststableat-20to-80degreesC,andcanbestoredforoneweekat2-8degreesC.FormaximalstABIlity,apportionthereconstitutedpreparationintoworkingaliquotsandstoreat-20degreesCto-80degreesC.Avoidrepeatedfreeze/thawcycles.

Notes:

FGF4
FGF-4
fibroblastgrowthfactor4
HBGF-4
HBGF-4TransformingproteinKS3
heparinsecretorytransformingprotein1
Heparinsecretory-transformingprotein1
Heparin-bindinggrowthfactor4
HST-1
HST-1HSTF-1
HSTF1fibroblastgrowthfactor4spliceisoform
HSTFGF-4
humanstomachcancer,transformingfactorfromFGF-relatedoncogene
kaposisarcomaoncogene
KFGF
K-FGF
KS3
oncogeneHST

AlternateNamesfor53BP1Antibody:

FGF4,alsonamedFGFKandKFGF,belongstothefibroblastgrowthfactor(FGF)family.BysignalingthroughtheFGFR1c,2c,3cand4receptors,FGF4hasfunctionsthatmaintainapopulationofProgenitorcellsintheepIBLastthatgeneratesmesoderm,andcontributetothestemcellpopulationthatisincorporatedinthetailbud.Itisalsorequiredforaxialelongationofthemouseembryoaftergastrulation.MaturehumanFGF4(71-206a.a.)shares91%,82%,94%and91%a.a.identitywithmurine,rat,canineandbovineFGF4.Additionally,FGF4sharesabout30%sequenceidentitywiththeprototypicalmembersoftheFGFfamily.)

Background:

Thisproductisforresearchuseonlyandisnotapprovedforuseinhumansorinclinicaldiagnosis.Peptidesandproteinsareguaranteedfor2yearsfromdateofreceipt.

Limitations:

FGF4,alsonamedFGFKandKFGF,belongstothefibroblastgrowthfactor(FGF)family.BysignalingthroughtheFGFR1c,2c,3cand4receptors,FGF4hasfunctionsthatmaintainapopulationofprogenitorcellsintheepiblastthatgeneratesmesoderm,andcontributetothestemcellpopulationthatisincorporatedinthetailbud.Itisalsorequiredforaxialelongationofthemouseembryoaftergastrulation.MaturehumanFGF4(71-206a.a.)shares91%,82%,94%and91%a.a.identitywithmurine,rat,canineandbovineFGF4.Additionally,FGF4sharesabout30%sequenceidentitywiththeprototypicalmembersoftheFGFfamily.)

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