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The introduction of transgenes into stem cells has shown to be a valuable experiment technique for studying stem cell biology. Transfecting stem cells without inhibiting cell viability and cell growth has shown to be difficult. DNA-In® Stem Transfection Reagent offers a simple, robust and reproducible method for delivering DNA into a wide range of stem cells, including neural stem cells. Formulated and optimized specifically for embryonic and adult stem cells, DNA-In® Stem is a new-generation transfection reagent that enables high efficiency transfection while maintaining maximum cell viability and cell growth.
Optimized formulation for DNA delivery into primary and iPS cells.
Maximum transfection efficiency and cell viability.
Requires only low amounts of DNA for maximum expression and exceptionally low toxicity.
Robust, reproducible performance across a wide range of DNA concentrations.
Learn about High Efficiency CRISPR-Cas9 Delivery in Stem Cells withEditPro™ Stem
Learn also about the new DNA-In® CRISPR transfection reagent for primary cells
Figure 1. Data above show cells transfected with DNA-In® Stem Transfection Reagent. Cells were plated in 24-well plates to give 60-70% confluency on the day of transfection. Cells were transfected with various amounts of a DNA plasmid, containing eGFP behind an EF1-alpha promoter using 1µl DNA-In® Stem. Cells were analyzed 24-hours and 48-hours (not shown) after transfection. Above, representative images of normal human induced pluripotent stem cells (iPSC)-derived neural stem cells (NSC), Human embryonic stem cells, adipose-derived mesenchymal stem cells (MSC) and Huntington's Disease (HD)-specific iPSCs show consistently high efficiency with low toxicity when using DNA-In® Stem transfection reagent.
Figure 2 (a-d). DNA-In® Stem requires up to 4X less DNA vs competitor reagents for maximum performance. Human iPSC-derived neural stem cells (NSC), Human embryonic stem cells, adipose-derived Mesencymal Stem Cells and Huntington's Disease (HD)-specific iPSCs plated in 24-well plates were transfected with DNA-In® Stem, Lipofectamine®2000 (L2000) and 3000 (L3000) using various amounts (µl) of reagent. Cells transfected with DNA-In® Stem were transfected with both low and high amounts of DNA. Cells were also transfected with L2000 and L3000 using their recommended 500ng of DNA.
Consistent, robust performance
Figure 3 (a-d). DNA-In® Stem shows robust performance across a range of DNA amounts in stem cells to product high transfection efficiency. Stem cells, including plated in 24-well plates were transfected with various amounts of DNA-In® Stem and DNA. Cells were then observed 24-hours (above data) and 48-hours (not shown) post-transfection. Above data for Human iPSC-derived neural stem cells (NSC), Human embryonic stem cells, adipose-derived Mesencymal Stem Cells and Huntington's Disease (HD)-specific iPSCs show consistently high efficiency with low toxicity when using DNA-In® Stem Transfection Reagent.