DNAIn® Stem Transfection Reagent的授权代理商

DNA-In® Stem TransfectionReagent

OptimizedformaximumDNAdeliveryinStemCells 

TheintroductionoftransgenesintostemcellshasshowntobeavaluableexperimenttechniqueforstudyingstemcellBIOLOGy.TransfectingstemcellswithoutinhibitingcellviABIlityandcellgrowthhasshowntobedifficult.DNA-In® StemTransfectionReagentoffersasimple,robustandreproducIBLemethodfordeliveringDNAintoawiderangeofstemcells,includingneuralstemcells.Formulatedandoptimizedspecificallyforembryonicandadultstemcells,DNA-In® Stemisanew-generationtransfectionreagentthatenableshighefficiencytransfectionwhilemaintainingmaximumcellviabilityandcellgrowth. 

• OptimizedformulationforDNAdeliveryintoprimaryandiPScells.

• Maximumtransfectionefficiencyandcellviability.

• RequiresonlylowamountsofDNAformaximumexpressionandexceptionallylowtoxicity.

• Robust,reproducibleperformanceacrossawiderangeofDNAconcentrations.

  LearnaboutHighEfficiencyCRISPR-Cas9DeliveryinStemCellswithEditPro™Stem

  Learnalsoaboutthenew DNA-In®CRISPR transfectionreagentforprimarycells

  Highefficiency,lowcytotoxicity

   

  

  Figure1. DataaboveshowcellstransfectedwithDNA-In® StemTransfectionReagent.Cellswereplatedin24-wellplatestogive60-70%confluencyonthedayoftransfection.CellsweretransfectedwithvariousamountsofaDNAplasmid,containingeGFPbehindanEF1-alphapromoterusing1µlDNA-In® Stem.Cellswereanalyzed24-hoursand48-hours(notshown)aftertransfection. Above,representativeimagesofnormalhumaninducedpluripotentstemcells (iPSC)-derivedneuralstemcells(NSC),Humanembryonicstemcells,ADIpose-derivedmesenchymalstemcells(MSC)andHuntington'sDisease(HD)-specificiPSCsshowconsistentlyhighefficiencywithlowtoxicitywhenusingDNA-In® Stemtransfectionreagent.

  Outperformscompetitorsacrossarangeofcelltypes

  

  Figure2(a-d). DNA-In® Stemrequiresupto4XlessDNAvscompetitorreagentsformaximumperformance.  HumaniPSC-derivedneuralstemcells(NSC),Humanembryonicstemcells,adipose-derivedMesencymalStemCellsandHuntington'sDisease(HD)-specificiPSCsplatedin24-wellplatesweretransfectedwithDNA-In® Stem,Lipofectamine®2000(L2000)and3000(L3000)usingvariousamounts(µl)ofreagent. CellstransfectedwithDNA-In® StemweretransfectedwithbothlowandhighamountsofDNA.CellswerealsotransfectedwithL2000andL3000usingtheirrecommended500ngofDNA.

  Consistent,robustperformance

  

  

  Figure3(a-d).DNA-In®StemshowsrobustperformanceacrossarangeofDNAamountsinstemcellstoproducthightransfectionefficiency. Stemcells,includingplatedin24-wellplatesweretransfectedwithvariousamountsofDNA-In®StemandDNA.Cellswerethenobserved24-hours(abovedata)and48-hours(notshown)post-transfection.AbovedataforHumaniPSC-derivedneuralstemcells(NSC),Humanembryonicstemcells,adipose-derivedMesencymalStemCellsandHuntington'sDisease(HD)-specificiPSCsshowconsistentlyhighefficiencywithlowtoxicitywhenusingDNA-In®StemTransfectionReagent.