Click ChemistryDBCO Reagents（材料英文详细介绍和应用）

西安瑞禧生物科技有限公司提供Clickchemistry-DBCOReagents（材料英文详细介绍和应用）相关资料如下：

名称：DBCO-NHSEster  MW：402.40
英文介绍：
DBCO-NHSEsterreactswithprimaryamines
Thisproductisnotrecommendedforlabelingofproteinsoranyotherbiopolymersinaqueousbuffersduetopooraqueoussolubility.
 
名称：PhotocleavableDBCO-NHSester MW:945.96
英文介绍：
Anamine-reactive,photocleavablelabelingreagent.
PhotocleavableDBCO-NHSesterreagentcontainsanaminereactivegrouplinkedtoDBCOmoietythroughaspacerarmcontainingaphotocleavablemoiety.Moleculesjointedbythecopper-freeclickreactioncanbeefficientlyphotoreleased,typically>90%in5-25minutesusinganinexpensive,near-UV,lowintensitylamp(e.g.365nmlampat1-5mW/cm2).
 
名称:DBCO-C6-NHSEster  MW:430.45
英文介绍：
Anactivatedesterwithextended6-carbonatomlongspacer.
DBCO-NHSEsterreactswithprimaryamines(e.g.,sidechainoflysineresiduesoraminosilane-coatedsurfaces)atpH7-9toformcovalentbonds.Extended6-carbonspacerarmisbeneficialforsomeapplication.Thisreagenthaslimitedshelflife;however,correspondingacidanditsderivativesarefoundtobestableoverlongperiodoftime.DBCO-Sulfo-NHS-Ester,awater-solubleversionofthisproductisalsoavailableforlabelingexperimentswherethepresenceoforganicsolventsishighlyundesirable.
 
名称：DBCO-PEG5-NHSEster MW:693.74
英文简介：
Anamine-reactivelabelingreagentwithenhancedwaterstABIlityandsolubility.
DBCO-PEG5-NHSEsterreactsspecificallyandefficientlywithaprimaryamine(e.g.,sidechainoflysineresiduesoraminosilane-coatedsurfaces)atpH7-9toformacovalentbond.Thehydrophilicpolyethyleneglycol(PEG)spacerarmimpartswatersolubilitythatistransferredtothelabeledmolecule,thusreducingaggregationoflabeledproteinsstoredinsolution.ThePEGspacerarmprovidesalongandflexIBLeconnectionthatminimizessterichindranceinvolvedwithligationtocomplementaryazide-containingmolecules.
 
名称：DBCO-PEG4-NHSEster MW:649.68
英文简介：
Anamine-reactivelabelingreagentwithenhancedwatersolubilityprovidedbyaPEG4spacer.
DBCO-PEG4-NHSEsterreactsspecificallyandefficientlywithaprimaryamine(e.g.,sidechainoflysineresiduesoraminosilane-coatedsurfaces)atpH7-9toformacovalentbond.Thehydrophilicpolyethyleneglycol(PEG)spacerarmimpartswatersolubilitythatistransferredtothelabeledmolecule,thusreducingaggregationoflabeledproteinsstoredinsolution.ThePEGspacerarmprovidesalongandflexibleconnectionthatminimizessterichindranceinvolvedwithligationtocomplementaryazide-containingmolecules.
 
名称：DBCO-Sulfo-NHSEster MW:532.50
英文简介：
Awater-solublereagentforlabelingantibodies,proteinsandothermoleculescontainingprimaryamines.
DBCO-Sulfo-NHS-Esterenablessimpleandefficientlabelingofantibodies,proteinsandanyotherprimaryamine-containingmacromoleculeswithDBCOmoiety.Thisreagentiswater-soluble,enablinglabelingtobeperformedintheabsenceoforganicsolventssuchasDMSOorDMFforapplicationsthatcannottoleratesolventsorarecomplicatedbytheirinclusion.Specificlabelingofcellsurfaceproteinsisanothercommonapplicationfortheseuniquelywater-solubleandmembraneimpermeablereagents.
 
名称：DBCO-Acid  MW:305.11
英文简介：
DBCO-Acidisanaminereactive,non-activatedcyclooctynebuildingblock.
Theuseofstrainedcyclooctynesinsteadofterminalalkynesallowsforovercomingamajordrawbackofcopper-catalyzedazide-alkynecycloaddition(CuAAC)-theneedforthetoxiccoppercatalyst.Theringstrainincyclooctynes,suchasDBCO-Acidmakesthemhighlyreactiveinthecycloadditionreaction.Thisreactionwastermedasthestrain-promotedalkyne-azidecycloaddition(SPAAC)orcopper-freeclickreactionandhasbeenwidelyusedforbioconjugationandforlabelinglivingcellsandlivinganimals.
 
名称：DBCO-C6-Acid  MW:333.83
英文简介：
AnanalogofDBCO-Acidwithimprovedstabilityandsolubilityincommonlyusedorganicsolvents.
DBCOreagents,alsoknownasADIBOorDIBACareroutinelyusedbuildingblocksforsynthesisofcomplexmoleculestobeusedinCu-freeligationapplications.
UsingDBCO-C6-AcidprimaryorsecondaryaminescanbeeasilyfunctionalizedwithDBCOmoietyinthepresenceofactivators(e.g.EDC,orHATU)throughastableamidebond.Alongercarbonspacerarmimprovesstabilityandsolubilityofthisreagentincommonlyusedorganicsolvents.
 
名称：DBCO-PEG5-Acid   MW:596.67
英文简介：
Anamine-reactivebuildingblockwithenhancedstabilityandsolubilityinaqueousmedia.
DBCO-PEG5-Acidisapolyethyleneglycol-containingreagentwithterminalcarboxylicacid(–COOH)thatcanbereactedwithprimaryorsecondaryaminegroupsinthepresenceactivators(e.g.EDC)formingastableamidebond.ThePEGspacerarmishydrophilicandprovidesgreatersolubilitytolabeledmoleculescomparedtoreagentshavingonlyhydrocarbonspacers.ThePEGspacerarmalsogivesthereagentalongandflexibleconnectiontominimizesterichindranceinvolvedwithreactingwithcomplementaryazido-modifiedmolecules.
 
名称：DBCO-PEG4-Acid MW:552.62
英文简介：
Anamine-reactivebuildingblockwithenhancedsolubilityinaqueousmedia.
DBCO-PEG4-Acidisapolyethyleneglycol-containingreagentwithterminalcarboxylicacid(–COOH)thatcanbereactedwithprimaryorsecondaryaminegroupsinthepresenceactivators(e.g.EDC)formingastableamidebond.ThePEGspacerarmishydrophilicandprovidesgreatersolubilitytolabeledmoleculescomparedtoreagentshavingonlyhydrocarbonspacers
 
名称：DBCO-PEG4-4-Formylbenzoate   MW:655.77
英文简介：
Aheterobifunctionalreagentforlabelingaminooxy-containingcompounds.
DBCO-PEG4-4-FormylbenzoatereactsspecificallyandefficientlywithanaminooxygroupatpH4-5toformacovalentoximebond.Theoximeligationwitharomaticaldehydesishighlychemoselectiveandiscompatiblewithotherfunctionalgroupspresentinpeptidesorbiomolecules.Ananilinecatalysiscanbeusedtoactivatearomaticaldehydetowardsanaminooxygroupandgreatlyenhancetherateofligationreaction.
 
DBCOReagents-Carboxyl/CarbonylReactive产品详细英文介绍和使用说明：
名称：DBCO-Amine  MW:276.33  CAS:1255942-06-3
英文简介：
Acarboxyl-reactivebuildingblockcontainingDBCOmoietyandaterminalprimaryaminegroup.
DBCO-AmineisasimpleDBCO-containingreagentpossessingaterminalprimaryaminesgroup(–NH2)thatreactswithcarboxylgroupsinthepresenceofactivators(e.g.EDC,orDCC)orwithactivatedesters(e.g.NHSesters)formingstableamidebonds.Ashortspacerarmaddsminimalmasstomodifiedmolecules(273.3daltons).
 
名称：DBCO-PEG4-Amine  MW:523.62
英文简介：
Acarbonyl-reactivebuildingblockwithenhancedsolubilityinaqueousmedia.
DBCO-PEG4-Amineisapolyethyleneglycol-containingreagentwithterminalprimaryamines(–NH2)thatreactswithcarboxylgroupsinthepresenceofactivators(e.g.EDC,orDCC)orwithactivatedesters(e.g.NHSesters)formingstableamidebonds.Ahydrophilicspacerarmenhancessolubilityinaqueousbuffers.
 
名称：ThiOlinker-DBCO
英文简介：
Aunique,water-soluble,bis-alkylatingreagentthatallowsforsite-specificlabelingofantibodiesundermildaqueousconditions.
Thetraditionalsulfhydryllabelingchemistrycleavesdisulfidebridgesandleavesthemin“openform”,whichmaycompromisethestabilityandfunctionofanantibodyorotherproteins.ThioLinkerTM-DCBOre-bridgesthedisulfidebond,andenhancesthestructuralstabilityoftheprotein.Inaddition,conjugatespreparedwithThioLinkerTMreagentsbenefitsfromreducedheterogeneityandbetterstability.
 
名称：DBCO-Maleimide MW:427.75
英文简介：
Ashort-length,sulfhydryl-reactivelabelingreagentthatcontainsashorthydrocarbonspacer.
DBCO-MaleimideenablessimpleandefficientincorporationofDBCOmoietyontocysteine-containingpeptidesorotherthiol-containingmolecules.Themaleimidegroupspecificallyandefficientlyreactswithreducedthiols(sulfhydrylgroups,–SH)atpH6.5to7.5toformstablethioetherbonds.Ashortspacerarmaddsminimalmasstomodifiedmolecules(427.4daltons).
 
名称：DBCO-PEG4-Maleimide MW:674.74
英文简介：
Amid-length,sulfhydryl-reactivelabelingreagentwithenhancedwatersolubilityprovidedbyaPEG4spacer.
DBCO-PEG4-MaleimideenablessimpleandefficientincorporationofDBCOmoietyontoantibodies,cysteine-containingpeptidesandotherthiol-containingmolecules.Thehydrophilicpolyethyleneglycol(PEG)spacerarmimpartswatersolubilitythatistransferredtothelabeledmolecule,thusreducingaggregationoflabeledproteinsstoredinthesolution.ThePEGspacerarmprovidesalongandflexibleconnectionthatminimizessterichindranceinvolvedwithligationtocomplementaryazide-containingmolecules.
 
名称：Bis-sulfone-PEG4-DBCO  MW：1006.19
英文简介：
Abis-alkylatinglabelingreagentusedtocreateconjugateswithenhancedstructuralstability.
Bis-sulfone-PEG4-DBCOenablessite-specificincorporationofDBCOmoietyontoantibodiestoformwelldefinedconjugates.Unlikeconjugatespreparedthroughconventional,maleimide-basedlabelingchemistrythatleavesdisulfidebridgesin“openform”andproducesaheterogeneousmixturelabeledspecieswithDAR1-8,conjugatespreparedwithbis-sulfonereagentsbenefitsfromreducedheterogeneityandenhancedstability.

名称：Bis-sulfone-PEG3-Azide MW:700.82
英文简介：
Abis-alkylatinglabelingreagentusedtocreatewell-definedconjugateswithenhancedstructuralstability.
Bis-sulfone-PEG3-Azideenablessite-specificincorporationofanazidegroupontoantibodiestoformwelldefinedconjugates.Unlikeconjugatespreparedthroughconventional,maleimide-basedlabelingchemistrythatleavesdisulfidebridgesin“openform”andproducesaheterogeneousmixturelabeledspecieswithDAR1-8,conjugatespreparedwithbis-sulfonereagentsbenefitsfromreducedheterogeneityandenhancedstability.
 
DBCOReagents-BifunctionalCrosslinkers产品详细英文介绍和使用说明：
名称：DBCO-PEG4-DBCO  MW:854.92
英文简介：
Anazide-to-azidehomobifunctionalcross-linkerwithahydrophilicPEG4spacerarm.
DBCO-PEG4-DBCOreactsspecificallyandefficientlywithanazidegroup(e.g.,asidechainofL-azidohomoalanine)toformastabletriazolemoiety.
 
名称：TCO-PEG12-DBCO
英文简介：
Anazide-to-tetrazineheterobifunctionalcross-linkerwithahydrophilicPEG12spacerarm.
Heterobifunctionalreagentsareusedforconvertingazido-containingbiomoleculesintoTCO-modifiedbiomolecules.TCO-PEG12-DBCOcanserveasaheterobifunctionalazide-to-tetrazinecross-linkerforcovalentlylinkingazido-andtetrazine-modifiedpeptidesorbiopolymers.ThePEG12spacerarmprovidesalongandflexibleconnectionthatminimizessterichindranceinvolvedwithligationtocomplementaryreagent.
 
名称：Methyltetrazine-DBCO
英文简介：
Awater-solubleazide-to-TCOheterobifunctionalcrosslinker.
Heterobifunctionalreagentsusedforconvertingazido-containingpeptidesorproteinintotetrazine-modifiedpeptidesorproteinwithouttheneedforcatalystoraxillaryreagents.Tetrazine-DBCOcanserveasaheterobifunctionalazide-to-TCOcross-linkerforcovalentlylinkingazido-andTCO-modifiedpeptidesorbiopolymers.
 
名称：Tetrazine-PEG12-DBCO
英文简介：
Anazide-to-TCOheterobifunctionalcrosslinkerwithahydrophilicPEG12spacerarm.
Heterobifunctionalreagentsusedforconvertingazido-containingpeptidesorproteinintotetrazine-modifiedpeptidesorproteinwithouttheneedforcatalystoraxillaryreagents.Tetrazine-PEG12-DBCOcanserveasaheterobifunctionalazide-to-TCOcross-linkerforcovalentlylinkingazido-andTCO-modifiedpeptidesorbiopolymers.
 
名称：DBCO-PEG4-4-Formylbenzoate
英文简介：
Aheterobifunctionalreagentforlabelingaminooxy-containingcompounds.
DBCO-PEG4-4-FormylbenzoatereactsspecificallyandefficientlywithanaminooxygroupatpH4-5toformacovalentoximebond.Theoximeligationwitharomaticaldehydesishighlychemoselectiveandiscompatiblewithotherfunctionalgroupspresentinpeptidesorbiomolecules.Ananilinecatalysiscanbeusedtoactivatearomaticaldehydetowardsanaminooxygroupandgreatlyenhancetherateofligationreaction.
 
名称：DBCO-PEG4-Biotin
英文简介：
Anazide-reactivebiotinylationreagentwithanextendedPEG4spacerarm.
DBCOreactswithanazidetoproduceastabletriazole,alsoreferredtoastheCu(I)‐freeorstrain-promotedclickreaction.Theazidegroup,whichisthereactivetargetofDBCO-activatedbiotin,canbeintroducedintoproteinsorothercellulartargetsviaazidelabelingreagentsorthroughinvivolabelingwithazide-derivativesofnaturallyoccurringmetabolicbuildingblocks.Thisreactionpossessesextremeselectivityandbiocompatibility,suchthatthecomplimentaryreagentscanformcovalentbondswithinrichlyfunctionalizedBIOLOGicalsystems,and,insomecases,livingorganisms.
 
名称：WSDBCO-Biotin
英文简介：
Awater-soluble,azide-reactivebiotinylationreagent.
DBCOreactswithanazidetoproduceastabletriazole,alsoreferredtoastheCu(I)‐freeorstrain-promotedclickreaction.Theazidegroup,whichisthereactivetargetofDBCO-activatedbiotin,canbeintroducedintoproteinsorothercellulartargetsviaazidelabelingreagentsorthroughinvivolabelingwithazide-derivativesofnaturallyoccurringmetabolicbuildingblocks.Thisreactionisextremelyselectiveandbiocompatible,suchthatthecomplimentaryreagentscanformacovalentbondwithinrichlyfunctionalizedbiologicalsystems,and,insomecases,livingorganisms.
 
名称：photoclevableDBCO-Biotin
英文简介：
Anazide-reactive,photoclevablebiotinprobethatallowsforreagent-freereleaseofthecapturedbiomoleculesfromstreptavidin.Extraordinarystrengthofthestreptavidin-biotininteractionallowsforefficientcapturingofevenhighlydilutetargets;however,itmakesrecoveryofproteinsfromaffinityresinschallenging.
 
名称：DBCO-PEG4-Desthiobiotin
英文简介：
Anazide-reactivedesthiobiotinreagentthatallowseasyelutionfromstreptavidin.
Desthiobiotinisasingle-ring,sulfur-freeanalogofbiotinthatbindstostreptavidinwithnearlyequalspecificitybutlessaffinitythanbiotin(Kd=1011vs.1015M,respectively).Consequently,desthiobiotinylatedproteinscanbeelutedreadilyandspecificallyfromstreptavidinaffinityresinusingmildconditionsbasedoncompetitivedisplacementwithfreebiotin.Forpull-downexperimentswithbiologicalsamples,thissoft-releasecharacteristicofdesthiobiotinalsohelpstominimizeco-purificationofendogenousbiotinylatedmolecules,whichremainboundtostreptavidinuponelutionofthetargetproteincomplexeswithfreebiotin.
 
名称：WS-PCDBCO-Biotin
英文简介：
Awater-soluble,photoclevablebiotinprobethatallowsforreagent-freereleaseofthecapturedbiomoleculesfromstreptavidin.
Extraordinarystrengthofthestreptavidin-biotininteractionallowsforefficientcapturingofevenhighlydilutetargets;however,itmakesrecoveryofproteinsfromaffinityresinschallenging.Photocleavablebiotinprobeseliminateamajorlimitationofthestreptavidin-biotinaffinitypurification.Thisreagentcontainsabiotinmoietylinkedtoanazidemoietythroughaspacerarmcontainingaphotocleavablemoiety.Capturedbiomoleculescanbeefficientlyphotoreleased,typically>90%in5-25minutesusinganinexpensive,near-UV,lowintensitylamp(e.g.365nmlampat1-5mW/cm2).
 
名称：DdeBiotin-DBCO
英文简介：
Ahydrazinecleavablebiotinylationreagentforselectiveenrichmentofazide-taggedbiopolymersviaSPAACreaction.
Extraordinarystrengthofthestreptavidin-biotininteractionallowsforefficientcapturingofevenhighlydilutetargets;however,itmakesrecoveryofproteinsfromaffinityresinschallenging.DdeBiotin-DBCOprobeeliminatesamajorlimitationofthestreptavidin-biotinaffinitypurification.ThisreagentcontainsabiotinmoietylinkedtoanalkynemoietythroughaspacerarmcontainingaDdelinker.Capturedbiomoleculescanbeefficientlyreleased,typically>90%.
 
名称：DiazoBiotin-DBCO
英文简介：
Asodiumdithionite(Na2S2O4)cleavablebiotinylationreagentforselectiveenrichmentofazide-taggedbiopolymersviaSPAACreaction.
DiazoBiotin-DBCOallowscaptureofazide-taggedbiomoleculesviatheCu-freeclickreactionundermildaqueousbufferconditions.Thisreactionpossessesextremebiocompatibility,suchthatthecomplementaryreagentscanformcovalentbondswithinlivingorganisms.Biotinylatedproteintargetscanbeselectivelycapturedthroughconventionalaffinitypurificationprotocolsthatexploitthebiotin-streptavidininteraction.Capturedproteintargetscanbereleasedundermildconditionswith50mMsodiumdithionitesolution,andidentifiedbymassspectrometry,ELISA,dotblot,orWesternblotapplications.
 
名称：DBCO-S-S-PEG3-Biotin
英文简介：
Acleavablebiotinylationreagentforspecificenrichmentazide-taggedbiomolecules.
DBCO-S-S-PEG3-Biotinenablessimpleandefficientbiotinlabelingofazide-taggedantibodies,proteinsandothermacromolecules.TheDBCOgroupreactswithanazidetoproduceanstabletriazole,whichisalsoreferredtoastheCu(I)-freeorstrain-promotedclickreaction.ThehydrophilicPEGspacerarmimpartswatersolubilityandreducesaggregationoflabeledproteinsstoredinthesolution.TheintegraldisulfidebondenablesthebiotingrouptobecleavedfromthelabeledmoleculeusingDTTorotherreducingagents.
 
名称：Carboxyrhodamine110DBCO
英文简介：
Agreenfluorescence-emittingprobewithexcitation/emissionmaximumof501/523nm
DBCO-activatedCarboxyrhodamine110dyereactswithazidestoproduceastabletriazole,alsoreferredtoastheCu-freeclickreaction,anddoesnotrequireCu-catalystorelevatedtemperatures.Thisreactionisextremelyselectiveandbiocompatible,suchthatthecomplimentaryreagentscanformacovalentbondwithinrichlyfunctionalizedbiologicalsystems,and,insomecases,livingorganisms.
 
名称：SulforhodamineBDBCO
英文简介：
Aredfluorescence-emittingprobewithexcitation/emissionmaximumof568/585nm
DBCO-activatedSulforhodamineBdyereactswithazidestoproduceastabletriazole,alsoreferredtoastheCu-freeclickreaction,anddoesnotrequireaCu-catalystorelevatedtemperatures.Thisreactionpossessesextremeselectivityandbiocompatibility,suchthatthecomplimentaryreagentscanformcovalentbondswithinrichlyfunctionalizedbiologicalsystems,and,insomecases,livingorganisms.
 
名称：TAMRADBCO
英文简介：
Anorange-to-redfluorescence-emittingprobewithexcitation/emissionmaximumof554/567nm
DBCO-activatedTAMRAdyereactswithazidestoproduceastabletriazole,alsoreferredtoastheCu-freeclickreaction,anddoesnotrequireCu-catalystorelevatedtemperatures.Thisreactionpossessesextremeselectivityandbiocompatibility,suchthatthecomplimentaryreagentscanformcovalentbondswithinrichlyfunctionalizedbiologicalsystems,and,insomecases,livingorganisms.
 
名称：Sulforhodamine101DBCO
英文简介：
Aredfluorescence-emittingprobewithexcitation/emissionmaximumof586/601nm
DBCO-activatedSulforhodamine101dyereactswithazidestoproduceastabletriazole,alsoreferredtoastheCu-freeclickreaction,anddoesnotrequireaCu-catalystorelevatedtemperatures.Thisreactionisextremelyselectiveandbiocompatible,suchthatthecomplimentaryreagentscanformcovalentbondswithinrichlyfunctionalizedbiologicalsystems,and,insomecases,livingorganisms.
 
名称：Cy3DBCO
英文简介：
Anazide-reactiveCy3dyeforchemoselectivelabelinganddetectionofazido-taggedmoleculesviatheCu-freeclickreaction.
 
名称：Cy5DBCO
英文简介：
Awater-soluble,azide-reactiveCy5dyeforchemoselectivelabelinganddetectionofazido-taggedmoleculesviatheCu-freeclickreaction.
 
名称：Cy5.5DBCO
英文简介：
Anazide-reactive,watersolubleCy5.5dyeforchemoselectivelabelinganddetectionofazido-taggedmolecules.
 
名称：Cy7DBCO
英文简介：
Anazide-reactive,water-solubleCy7dyeforchemoselectivelabelinganddetectionofazido-taggedmoleculesviatheCu-freeclickreaction.
 
名称：DBCO-PEG4-Phosphoramidite
英文简介：
DBCO-PEG4-PhosphoramiditeisareagentforincorporationofDBCOmoietyontoanoligonucleotideforsubsequentcopper-freeclickelaboration.
 
名称：DBCO-PEG4-Hydroxyl
英文简介：
Amodificationreagentcontainingalong4-unitpolyethyleneglycol(PEG4)spacerarmandaterminalprimaryhydroxylgroup.


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