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Reagentproteins/CRM197/CRM-197/1.00 mg

CatalogNumber 

CRM-197

Description 

CRM197diphtheriatoxinmutant,recombinant.Pre-clinicalgrade.

ProductBackground 

CRM197isanon-toxicmutantofdiphtheriatoxin1,2havingasingleaminoacidsubstitutionofglutamicacidforglycine.CRM197isawelldefinedproteinandfunctionsasacarrierforpolysaccharidesandhaptensmakingthemimmunogenic.Itisutilizedasacarrierproteininanumberofapprovedconjugatevaccinesfordiseasessuchasmeningitisandpneumococcalbacterialinfections.PfenexCRM197isarecombinantformexpressedinPseudomonasfluorescensusingthePf?nexExpressionTechnologyTMplatform.

Thisproductisavailableinbothpre-clinicalandcGMPgrade.

FormoreinformationonCRM197orourothervaccinecarriers,pleasecontactusorvisitourknowledgecenterforarticles,publicationsandotherresourcesonCRM197andourothervaccinecarriers.


 

Category 

VaccineComponents

Source 

Pseudomonasfluorescens

Species 

CoryNEBacteriumdiphtheriae

Formulation 

CRM197islyophilizedfroma0.2um-filteredsolutioncontaining4mg/mLCRM197,50mg/mLsucrose,0.22mg/mLsodiumphosphatemonobasic,3mg/mLsodiumphosphatedibasic,0.055mg/mLpolysorbate80.

ProductMolecularMass:58.4kDa

Storage 

Storeat2-8°Cuponreceipt

Purity 

Purity:>95%CRM197bySDS-PAGE
            AandBchaincontent<5%oftotalCRM197

Endotoxin:<100EU/mgofproteinbyLALmethod

Dimer:<5%

     

Uses 

Beforeuse,asepticallyreconstitutelyophilizedCRM197withsteriledeionizedwatertoaconcentrationof4mg/mLCRM197.ReconstitutedCRM197shouldbestoredat2-8&deg;C.
 

References 

1.Giannini,G.,Rappuoli,R.,andRatti,G.NucleicAcidsRes.12:4063-4069(1984)
2.Mekada,E.,andUchida,T.J.Biol.Chem.260:12148-12153(1985)
     

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