DNAIn® Neuro Transfection Reagent的授权代理商

DNA-In® Neuro TransfectionReagent

FormulatedSpecificallyforNeurons 

Developedbytheco-inventorsoftheearlyLipofectamine® products,DNA-In® NeuroTransfectionreagentwasformulatedfromanovelchemistryformaximumtransfectionefficiencyinneurons.Inside-by-sideassayswithtopcompetitorreagentssignificantlyhighertransfectionefficienciesareconsistentlyobservedwhenDNA-In® Neuroisusedto transfect primarycortical, hippocampal orforebrainneurons.Moreover,withlow cytotoxic effectDNA-In® Neurosupportsneuronalsurvivaland neurite extensionpost-transfection.

Figure1.High GFP ExpressioninPrimaryNeurons –PrimaryratcorticalneuronsweretransfectedwithDNA-In®NeuroTransfectionReagentandincubatedovernightincompleteculturemedia.Theaboveimagesweretaken48-hourspost-transfectionandshowuniform,highGFPexpressioninhealthycells.

FeatureBenefits:

• HigherTransfectionEfficiency -Two-foldorgreaterimprovementinefficiencyovercompetingtransfectionreagents

• ExceptionallyLowToxicity -Maximumpost-transfectionneuronviABIlitycriticalforperformingassaysonhealthy, uncompromisedtransfected neurons.

• HighlyRobustPerformance –ProducesconsistentandreproducIBLeresults.

• QuickandEasy-to-Use – Aneasytofollow,single-tubereagentprotocol forgreatresultsontheveryfirsttry.

   

  SUPERIORTRANSFECTIONEFFICIENCYvs.LEADINGCOMPETITORREAGENTS

  DNA-In® NeuroTransfectionReagent isanewtransfectionreagentthatconsistentlyproduceshightransfectionefficienciesinneurons,typicallyachievinga2-foldorbetterimprovementinefficiencyoverthecompetingreagentscurrentlyavailable,including Lipofectamine® 2000and NeuroFECT™.Moreover,DNA-In® Neuroenablesneuronstobeefficiently transfected withminimaltoxicitytosupporthealthy post-transfected neurons,criticalforperformingassayson uncompromised transfected cells. 

   

  

  

  Figure2.(Top,Bottom). DNA-In® NeuroOutperformsLeadingCompetitorReagent–DNA-In® NeurowasusedtotransfectplasmidDNAencodingGFPinto6-dayculturedE18PrimaryRatCorticalNeurons.Transfectionswereperformedin24-wellplatesusing1.0-3.0µlofDNA-In®NeuroReagent.TheabovedatashowDNA-In®NeurosignificantlyoutperformsLipofectamine®2000withnear3-foldimprovementintransfectionefficiencyafter24hours(BottomGraph).Duplicatewellswereassayed24-hrsand48-hrsaftertransfection.

  WESTERNBLOTANALYSISOFTRANSFECTEDCORTICALNEURONS

   

  Figure2. Westernblotanalysisofthe transfected neuronsusingDNA-INNeuro- Corticalneurons5daysinculturewere transfected with(1) p35and(2) p25 (pCDNA3.1(-) Myc HisA)plasmidsin6wellplates 3µg/wellandusing 12µL ofDNA-In®Neuroneurontransfectionreagent. After48hr,westernblotanalysiswasperformedusing 30µg ofcell lysates foreachplasmidtoshowexpression. Datacourtesy ofDr. NiranjanaAmin, NIH, NINDS.

   

  ROBUST,EASYTOUSE,ONE-TUBEREAGENT 

  

   

  MOREDNA-In® CELL-SPECIFICREAGENTS

  OurfullproductlineofDNA-In®Cell-SpecificTransfectionReagentsisoptimizedforexceptionallyhighefficiencyandcellviabilityperformanceinspecificcelllines. Thedosage,procedureandformulationofeachofthesereagentshasbeenextensivelytestedandvalidatedbyour MTI-GlobalStem developersandothercustomers.