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CatalogNumber
CRM-197
Description
CRM197diphtheriatoxinmutant,recombinant.Pre-clinicalgrade.
ProductBackground
CRM197isanon-toxicmutantofdiphtheriatoxin1,2 havingasingleaminoacidsubstitutionofglutamicacidforglycine.CRM197isawelldefinedproteinandfunctionsasacarrierforpolysaccharidesandhaptensmakingthemimmunogenic.Itisutilizedasacarrierproteininanumberofapprovedconjugatevaccinesfordiseasessuchasmeningitisandpneumococcalbacterialinfections.PfenexCRM197isarecombinantformexpressedinPseudomonasfluorescensusingthe Pf?nexExpressionTechnologyTM platform.
Thisproductisavailableinbothpre-clinicaland cGMPgrade.
FormoreinformationonCRM197orourothervaccinecarriers,please contactus orvisitour knowledgecenter for articles, publications andotherresourcesonCRM197andourothervaccinecarriers.
Category
VaccineComponents
Source
Pseudomonasfluorescens
Species
CoryNEBacteriumdiphtheriae
Formulation
CRM197islyophilizedfroma0.2um-filteredsolutioncontaining4mg/mLCRM197,50mg/mLsucrose,0.22mg/mLsodiumphosphatemonobasic,3mg/mLsodiumphosphatedibasic,0.055mg/mLpolysorbate80.
ProductMolecularMass:58.4kDa
Storage
Storeat2-8°Cuponreceipt
Purity
Purity:>95%CRM197bySDS-PAGE
AandBchaincontent<5%oftotalCRM197
Endotoxin:<100EU/mgofproteinbyLALmethod
Dimer:<5%
Uses
Beforeuse,asepticallyreconstitutelyophilizedCRM197withsteriledeionizedwatertoaconcentrationof4mg/mLCRM197.ReconstitutedCRM197shouldbestoredat2-8°C.
References
1.Giannini,G.,Rappuoli,R.,andRatti,G.NucleicAcidsRes.12:4063-4069(1984)
2.Mekada,E.,andUchida,T.J.Biol.Chem.260:12148-12153(1985)
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