蚂蚁淘提供DNAIn® Stem Transfection Reagent/代理/现货/报价

2016年10月16日报价: 666元/单 单位: 上海代理报关公司 姓名:钱经理 电话: 021-61060986 卓鹰进口物流项目,专精以“新旧机电、食品酒类、原木木材、化工原料、妆化保健、杂货原料”6大类货类共160...

　　DNA-In®StemTransfectionReagent

　　OptimizedformaximumDNAdeliveryinStemCells

　　TheintroductionoftransgenesintostemcellshasshowntobeavaluableexperimenttechniqueforstudyingstemcellBIOLOGy.TransfectingstemcellswithoutinhibitingcellviABIlityandcellgrowthhasshowntobedifficult.DNA-In®StemTransfectionReagentoffersasimple,robustandreproducIBLemethodfordeliveringDNAintoawiderangeofstemcells,includingneuralstemcells.Formulatedandoptimizedspecificallyforembryonicandadultstemcells,DNA-In®Stemisanew-generationtransfectionreagentthatenableshighefficiencytransfectionwhilemaintainingmaximumcellviabilityandcellgrowth.

　　OptimizedformulationforDNAdeliveryintoprimaryandiPScells.

　　Maximumtransfectionefficiencyandcellviability.

　　RequiresonlylowamountsofDNAformaximumexpressionandexceptionallylowtoxicity.

　　Robust,reproducibleperformanceacrossawiderangeofDNAconcentrations.

　　LearnaboutHighEfficiencyCRISPR-Cas9DeliveryinStemCellswithEditPro™Stem

　　LearnalsoaboutthenewDNA-In®CRISPRtransfectionreagentforprimarycells

　　Highefficiency,lowcytotoxicity

 

　　Figure1.DataaboveshowcellstransfectedwithDNA-In®StemTransfectionReagent.Cellswereplatedin24-wellplatestogive60-70%confluencyonthedayoftransfection.CellsweretransfectedwithvariousamountsofaDNAplasmid,containingeGFPbehindanEF1-alphapromoterusing1µlDNA-In®Stem.Cellswereanalyzed24-hoursand48-hours(notshown)aftertransfection.Above,representativeimagesofnormalhumaninducedpluripotentstemcells(iPSC)-derivedneuralstemcells(NSC),Humanembryonicstemcells,ADIpose-derivedmesenchymalstemcells(MSC)andHuntington'sDisease(HD)-specificiPSCsshowconsistentlyhighefficiencywithlowtoxicitywhenusingDNA-In®Stemtransfectionreagent.

　　Outperformscompetitorsacrossarangeofcelltypes

　　

 

　　Figure2(a-d).DNA-In®Stemrequiresupto4XlessDNAvscompetitorreagentsformaximumperformance.HumaniPSC-derivedneuralstemcells(NSC),Humanembryonicstemcells,adipose-derivedMesencymalStemCellsandHuntington'sDisease(HD)-specificiPSCsplatedin24-wellplatesweretransfectedwithDNA-In®Stem,Lipofectamine®2000(L2000)and3000(L3000)usingvariousamounts(µl)ofreagent.CellstransfectedwithDNA-In®StemweretransfectedwithbothlowandhighamountsofDNA.CellswerealsotransfectedwithL2000andL3000usingtheirrecommended500ngofDNA.

　　Consistent,robustperformance

　　

 

　　

 

　　Figure3(a-d).DNA-In®StemshowsrobustperformanceacrossarangeofDNAamountsinstemcellstoproducthightransfectionefficiency.Stemcells,includingplatedin24-wellplatesweretransfectedwithvariousamountsofDNA-In®StemandDNA.Cellswerethenobserved24-hours(abovedata)and48-hours(notshown)post-transfection.AbovedataforHumaniPSC-derivedneuralstemcells(NSC),Humanembryonicstemcells,adipose-derivedMesencymalStemCellsandHuntington'sDisease(HD)-specificiPSCsshowconsistentlyhighefficiencywithlowtoxicitywhenusingDNA-In®StemTransfectionReagent.

300)this.width=300" border=0 alt="上海洋山进口代理清关公司 价格:666元/单">卓鹰进口物流项目，专精以“新旧机电、食品酒类、原木木材、化工原料、妆化保健、杂货原料”6大类货类共1600多种商品为细分物流服务，力求精准高效地服务于客户。目前，卓鹰以“三点一线（即卖点、买点、航线及操作点）”的标准整合了海内外优质物流配套使用资源，在沿海主要进口枢纽港，依据不同产品需求整合了优质物流资源，涵括船公司、关务、仓储、保***区服务、进出口权、行业资质许可、货物金融服务、物流配送等物流资源。~~~~~~~~~~~~~~~~~~~~~~~~~~~~~「上海卓鹰进出口有限公司」service center服务中心——进口报关总部（中国自贸区）联系人：钱经理（进口清关部）手机：15000636740座机：021-61060986~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~卓鹰进口物流，以提供热情、创新与专业的服务为营运宗旨，坚信“公司有多大，社会责任就有多大”的经营理念。卓鹰物流方案，均以“综合物流方案专家”的身份及“顾问专属秘书服务”的理念服务呈现于每一位客户。公司一直关注并加强电子商务化物流体系，提升服务水平及提高服务效率。因此，卓鹰创建了专属的“ZOUIN物流供应链”管理系统，系统涵括“数据中心、操作数控中心、物资(物流资源)数控中心、财控数控中心、案例数据中心及系统监控中心”等多个模块，建立“标准进口物流”服务体系。卓鹰多元差异化服务。为满足货主的不同需求，力求更具有优势性的物流方案，针对不同产品，不同口岸进口，在航线、船东、码头、关务等物流环节提供专业指导建议，同时，提供多元化的物流方案。卓鹰一直在拓展国际物流供应链多元化的综合业务统筹范畴，努力打造一个更加优质的服务平台。规格： 上海数量：100包装：ZOUIN日期：2016-10-16